ArticleThe Journal of cell biology2015
A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Dual Roads of CENP-A for Centromere Identification.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- The dynamics of centromere assembly and disassembly during quiescence.The Journal of cell biology · 2026Article
- Phosphorylation of Xenopus M18BP1 governs centromeric localization and CENP-A nucleosome assembly.EMBO reports · 2026Article
- The dynamics of centromere assembly and disassembly during quiescence.bioRxiv : the preprint server for biology · 2025Article
- Centromeric localization of αKNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions.Nucleic acids research · 2025Article
- RAD52-mediated repair of DNA double-stranded breaks at inactive centromeres leads to subsequent apoptotic cell death.Nucleic acids research · 2024Article
- Native and tagged CENP-A histones are functionally inequivalent.Epigenetics & chromatin · 2024Article
- Quantitative Super-Resolution Microscopy Reveals the Relationship between CENP-A Stoichiometry and Centromere Physical Size.International journal of molecular sciences · 2023Article
- Repression of CENP-A assembly in metaphase requires HJURP phosphorylation and inhibition by M18BP1.The Journal of cell biology · 2023Article
- The cryo-EM structure of the CENP-A nucleosome in complex with ggKNL2.The EMBO journal · 2023Article
- Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease.bioRxiv : the preprint server for biology · 2023Article
- Histone variants and chromatin structure, update of advances.Computational and structural biotechnology journal · 2023Review
- Molecular Complexes at Euchromatin, Heterochromatin and Centromeric Chromatin.International journal of molecular sciences · 2021Review
- De novo reconstitution of chromatin using wheat germ cell-free protein synthesis.FEBS open bio · 2021Article
- Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.Nature communications · 2019Article
- CDK phosphorylation ofThe EMBO journal · 2019Article
- Centromere and Pericentromere Transcription: Roles and Regulation … in Sickness and in Health.Frontiers in genetics · 2018Review
- Review
- Xenopus laevis M18BP1 Directly Binds Existing CENP-A Nucleosomes to Promote Centromeric Chromatin Assembly.Developmental cell · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.