ArticlePloS one2015
High-Throughput Sequencing Identifies Novel and Conserved Cucumber (Cucumis sativus L.) microRNAs in Response to Cucumber Green Mottle Mosaic Virus Infection.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Decoding MicroRNA-Guided Antiviral Defense in Cucurbitaceae: Regulatory Networks, RNA Silencing Cross-Talk, and Emerging Strategies for Crop Resilience.International journal of molecular sciences · 2026Review
- Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions.Stress biology · 2022Review
- Exploring the Effect of Methyl Jasmonate on the Expression of microRNAs Involved in Biosynthesis of Active Compounds of Rosemary Cell Suspension Cultures through RNA-Sequencing.International journal of molecular sciences · 2022Article
- Exogenous Application of dsRNA for the Control of Viruses in Cucurbits.Frontiers in plant science · 2022Article
- New early phenotypic markers for cucumber green mottle mosaic virus disease in cucumbers exposed to fluctuating extreme temperatures.Scientific reports · 2021Article
- Effects of Host-Adaptive Mutations on Hop Stunt Viroid Pathogenicity and Small RNA Biogenesis.International journal of molecular sciences · 2020Article
- Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana.Virology journal · 2019Article
- DNA Methylation Analysis of theGenes · 2019Article
- Identification of cucumber circular RNAs responsive to salt stress.BMC plant biology · 2019Article
- Expression profiling and regulatory network of cucumber microRNAs and their putative target genes in response to cucumber green mottle mosaic virus infection.Archives of virology · 2019Article
- Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive toInternational journal of molecular sciences · 2019Article
- High-throughput sequencing of small RNAs revealed the diversified cold-responsive pathways during cold stress in the wild banana (Musa itinerans).BMC plant biology · 2018Article
- Suppression of nbe-miR166h-p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation.Molecular plant pathology · 2018Article
- Down-regulation of genes coding for core RNAi components and disease resistance proteins via corresponding microRNAs might be correlated with successful Soybean mosaic virus infection in soybean.Molecular plant pathology · 2018Article
- Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress.PloS one · 2018Article
- Genome-wide identification of new reference genes for RT-qPCR normalization in CGMMV-infectedPeerJ · 2018Article
- Comparative analysis of miRNA and mRNA abundance in determinate cucumber by high-throughput sequencing.PloS one · 2018Article
- Transcriptome analysis of watermelon (Citrullus lanatus) fruits in response to Cucumber green mottle mosaic virus (CGMMV) infection.Scientific reports · 2017Article
- Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing.PloS one · 2017Article
- Prediction of Host-Derived miRNAs with the Potential to Target PVY in Potato Plants.Frontiers in genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Seedlings of Cucumis sativus L. (cv. 'Zhongnong 16') were artificially inoculated with Cucumber green mottle mosaic virus (CGMMV) at the three-true-leaf stage. Leaf and flower samples were collected at different time points post-inoculation (10, 30 and 50 d), and processed by high throughput sequencing analysis to identify candidate miRNA sequences. Bioinformatic analysis using screening criteria, and secondary structure prediction, indicated that 8 novel and 23 known miRNAs (including 15 miRNAs described for the first time in vivo) were produced by cucumber plants in response to CGMMV infection. Moreover, gene expression profiles (p-value <0.01) validated the expression of 3 of the novel miRNAs and 3 of the putative candidate miRNAs and identified a further 82 conserved miRNAs in CGMMV-infected cucumbers. Gene ontology (GO) analysis revealed that the predicted target genes of these 88 miRNAs, which were screened using the psRNATarget and miRanda algorithms, were involved in three functional categories: 2265 in molecular function, 1362 as cellular components and 276 in biological process. The subsequent Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the predicted target genes were frequently involved in metabolic processes (166 pathways) and genetic information processes (40 pathways) and to a lesser degree the biosynthesis of secondary metabolites (12 pathways). These results could provide useful clues to help elucidate host-pathogen interactions in CGMMV and cucumber, as well as for the screening of resistance genes.
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