ArticlePloS one2015
A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
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- Genetic impairment of folate metabolism regulates cortical interneurons and social behavior.Frontiers in neuroscience · 2023Article
- Application of the mirror technique for block-face scanning electron microscopy.Brain structure & function · 2022Article
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- Determining the optimal expression method for dual-color imaging.Journal of neuroscience methods · 2021Article
- Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor.PloS one · 2021Article
- Correction of z-motion artefacts to allow population imaging of synaptic activity in behaving mice.The Journal of physiology · 2020Article
- The Emergence of a Stable Neuronal Ensemble from a Wider Pool of Activated Neurons in the Dorsal Medial Prefrontal Cortex during Appetitive Learning in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- A unique olfactory bulb microcircuit driven by neurons expressing the precursor to glucagon-like peptide 1.Scientific reports · 2019Article
- Inhibitory interneurons mediate autism-associated behaviors via 4E-BP2.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- GABA-Glycine Cotransmitting Neurons in the Ventrolateral Medulla: Development and Functional Relevance for Breathing.Frontiers in cellular neuroscience · 2019Article
- Acquired parvalbumin-selective interneuronopathy in the multiple-hit model of infantile spasms: A putative basis for the partial responsiveness to vigabatrin analogs?Epilepsia open · 2018Article
- A neuronal role of the Alanine-Serine-Cysteine-1 transporter (SLC7A10, Asc-1) for glycine inhibitory transmission and respiratory pattern.Scientific reports · 2018Article
- Adult Born Periglomerular Cells of Odorant Receptor Specific Glomeruli.Frontiers in neuroanatomy · 2018Article
- Cell Type-Dependent Activation Sequence During Rhythmic Bursting in the PreBötzinger Complex in Respiratory Rhythmic Slices From Mice.Frontiers in physiology · 2018Article
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- Structural Features of an OR37 Glomerulus: A Comparative Study.Frontiers in neuroanatomy · 2017Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GABAergic inhibitory neurons are a large population of neurons in the central nervous system (CNS) of mammals and crucially contribute to the function of the circuitry of the brain. To identify specific cell types and investigate their functions labelling of cell populations by transgenic expression of fluorescent proteins is a powerful approach. While a number of mouse lines expressing the green fluorescent protein (GFP) in different subpopulations of GABAergic cells are available, GFP expressing mouse lines are not suitable for either crossbreeding to other mouse lines expressing GFP in other cell types or for Ca2+-imaging using the superior green Ca2+-indicator dyes. Therefore, we have generated a novel transgenic mouse line expressing the red fluorescent protein tdTomato in GABAergic neurons using a bacterial artificial chromosome based strategy and inserting the tdTomato open reading frame at the start codon within exon 1 of the GAD2 gene encoding glutamic acid decarboxylase 65 (GAD65). TdTomato expression was observed in all expected brain regions; however, the fluorescence intensity was highest in the olfactory bulb and the striatum. Robust expression was also observed in cortical and hippocampal neurons, Purkinje cells in the cerebellum, amacrine cells in the retina as well as in cells migrating along the rostral migratory stream. In cortex, hippocampus, olfactory bulb and brainstem, 80% to 90% of neurons expressing endogenous GAD65 also expressed the fluorescent protein. Moreover, almost all tdTomato-expressing cells coexpressed GAD65, indicating that indeed only GABAergic neurons are labelled by tdTomato expression. This mouse line with its unique spectral properties for labelling GABAergic neurons will therefore be a valuable new tool for research addressing this fascinating cell type.
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