ArticleBioconjugate chemistry2015
Synthetically Modified Viral Capsids as Versatile Carriers for Use in Antibody-Based Cell Targeting.
Article in Bioconjugate chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Tailored Viral-like Particles as Drivers of Medical Breakthroughs.International journal of molecular sciences · 2024Review
- RNA and Single-Stranded DNA Phages: Unveiling the Promise from the Underexplored World of Viruses.International journal of molecular sciences · 2023Review
- Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein-Protein Conjugation Strategy.Journal of the American Chemical Society · 2023Article
- Strategies for Multienzyme Assemblies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells.Scientific reports · 2021Article
- Synthesis at the interface of virology and genetic code expansion.Current opinion in chemical biology · 2018Review
- Extravasation of Brownian Spheroidal Nanoparticles through Vascular Pores.Biophysical journal · 2018Article
- Biologically-derived nanomaterials for targeted therapeutic delivery to the brain.Science progress · 2018Review
- Review
- Article
- Plant viruses and bacteriophages for drug delivery in medicine and biotechnology.Current opinion in chemical biology · 2017Review
- Display of single-chain variable fragments on bacteriophage MS2 virus-like particles.Journal of nanobiotechnology · 2017Article
- Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles.Bioconjugate chemistry · 2016Article
- Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy.Chemical communications (Cambridge, England) · 2016Article
- Design of virus-based nanomaterials for medicine, biotechnology, and energy.Chemical Society reviews · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The present study describes an efficient and reliable method for the preparation of MS2 viral capsids that are synthetically modified with antibodies using a rapid oxidative coupling strategy. The overall protocol delivers conjugates in high yields and recoveries, requires a minimal excess of antibody to achieve modification of more than 95% of capsids, and can be completed in a short period of time. Antibody-capsid conjugates targeting extracellular receptors on human breast cancer cell lines were prepared and characterized. Notably, conjugation to the capsid did not significantly perturb the binding of the antibodies, as indicated by binding affinities similar to those obtained for the parent antibodies. An array of conjugates was synthesized with various reporters on the interior surface of the capsids to be used in cell studies, including fluorescence-based flow cytometry, confocal microscopy, and mass cytometry. The results of these studies lay the foundation for further exploration of these constructs in the context of clinically relevant applications, including drug delivery and in vivo diagnostics.
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Registered trials
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