ArticlePloS one2015
Morphological and Gene Expression Changes in Cattle Embryos from Hatched Blastocyst to Early Gastrulation Stages after Transfer of In Vitro Produced Embryos.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.Frontiers in veterinary science · 2021Pooled it
- Pregnancy Outcomes Following Artificial Insemination and Embryo Transfer in Dairy Cows-Expectations vs. Reality.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Interleukin-6 and leukemia inhibitory factor differentially modify embryonic disk composition in post hatching bovine embryos†.Biology of reproduction · 2026Article
- Developmental origins of pregnancy loss in cattle.Animal reproduction · 2026Article
- A network of transient domains for breaking symmetry during anterior-posterior axis formation in the porcine embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2025Article
- Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation.BMC genomics · 2025Article
- Prenatal and Preweaning Environmental Effects upon Pubertal Development and Sperm Production.Advances in anatomy, embryology, and cell biology · 2025Review
- Enhancing integration of the dairy and beef sectors through application of assisted reproductive technologies: pregnancy outcomes following timed AI and timed ET in lactating dairy cows.Animal reproduction · 2025Article
- New findings on the orientation of the mouse anterior-posterior (A-P) axis before and during the initiation of gastrulation using a more refined embryo staging.Biochemistry and biophysics reports · 2024Article
- SMC2 ablation impairs bovine embryo development shortly after blastocyst hatching.Reproduction (Cambridge, England) · 2024Article
- Impact of Mycotoxin Metabolites Deepoxy-Deoxynivalenol and Beta-Zearalenol on Bovine Preimplantation Embryo Development in the Presence of Acetonitrile.Veterinary sciences · 2024Article
- Article
- Developmental progression continues during embryonic diapause in the roe deer.Communications biology · 2024Article
- Understanding bovine embryo elongation: a transcriptomic study of trophoblastic vesicles.Frontiers in physiology · 2024Article
- Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle.Animals : an open access journal from MDPI · 2023Review
- The evolution of gastrulation morphologies.Development (Cambridge, England) · 2023Review
- Alternative mammalian strategies leading towards gastrulation: losing polar trophoblast (Rauber's layer) or gaining an epiblast cavity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- In vitro culture of ovine embryos up to early gastrulating stages.Development (Cambridge, England) · 2022Article
- Expression patterns of signalling molecules and transcription factors in the early rabbit embryo and their significance for modelling amniote axis formation.Development genes and evolution · 2021Article
- A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.Journal of developmental biology · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A detailed morphological staging system for cattle embryos at stages following blastocyst hatching and preceding gastrulation is presented here together with spatiotemporal mapping of gene expression for BMP4, BRACHYURY, CERBERUS1 (CER1), CRIPTO, EOMESODERMIN, FURIN and NODAL. Five stages are defined based on distinct developmental events. The first of these is the differentiation of the visceral hypoblast underlying the epiblast, from the parietal hypoblast underlying the mural trophoblast. The second concerns the formation of an asymmetrically positioned, morphologically recognisable region within the visceral hypoblast that is marked by the presence of CER1 and absence of BMP4 expression. We have termed this the anterior visceral hypoblast or AVH. Intra-epiblast cavity formation and the disappearance of the polar trophoblast overlying the epiblast (Rauber's layer) have been mapped in relation to AVH formation. The third chronological event involves the transition of the epiblast into the embryonic ectoderm with concomitant onset of posterior NODAL, EOMES and BRACHYURY expression. Lastly, gastrulation commences as the posterior medial embryonic ectoderm layer thickens to form the primitive streak and cells ingress between the embryonic ectoderm and hypoblast. At this stage a novel domain of CER1 expression is seen whereas the AVH disappears. Comparison with the mouse reveals that while gene expression patterns at the onset of gastrulation are well conserved, asymmetry establishment, which relies on extraembryonic tissues such as the hypoblast and trophoblast, has diverged in terms of both gene expression and morphology.
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