ArticleMolecular microbiology2015
Transcriptional regulation of the Chlamydia heat shock stress response in an intracellular infection.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Genome-Wide Quantification of Protein-DNA Interactions in Chlamydia Using ChIP-Seq (Chromatin Immunoprecipitation/Deep Sequencing).Methods in molecular biology (Clifton, N.J.) · 2027Article
- Iron limitation-induced modulation of transcription in Chlamydia trachomatis.Scientific reports · 2026Article
- Late gene regulation by the alternative sigma factors ofmSystems · 2025Article
- A lineage-specific heat-induced feedback loop controls HrcA to promote chlamydial fitness under stress.bioRxiv : the preprint server for biology · 2025Article
- A conservedMicrobiology (Reading, England) · 2025Article
- Requirement of GrgA formBio · 2024Article
- Requirement of GrgA forbioRxiv : the preprint server for biology · 2023Article
- Chlamydia trachomatis RsbU Phosphatase Activity Is Inhibited by the Enolase Product, Phosphoenolpyruvate.Journal of bacteriology · 2022Article
- Article
- Robust Heat Shock Response inFrontiers in microbiology · 2021Article
- Article
- The Repressor Function of theJournal of bacteriology · 2020Article
- Protein aggregation in bacteria.FEMS microbiology reviews · 2020Review
- Using Intra-ChIP to Measure Protein-DNA Interactions in Intracellular Pathogens.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Biochemical and Genetic Analysis of the Chlamydia GroEL Chaperonins.Journal of bacteriology · 2017Article
- Tryptophan Codon-Dependent Transcription in Chlamydia pneumoniae during Gamma Interferon-Mediated Tryptophan Limitation.Infection and immunity · 2016Article
- Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum.The ISME journal · 2016Article
- Intra-ChIP: studying gene regulation in an intracellular pathogen.Current genetics · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacteria encode heat shock proteins that aid in survival during stressful growth conditions. In addition, the major heat shock proteins of the intracellular bacterium Chlamydia trachomatis have been associated with immune pathology and disease. We developed a ChIP-qPCR method to study the regulation of chlamydial heat shock gene regulation during an intracellular infection. This approach allowed us to show that chlamydial heat shock genes are regulated by the transcription factor HrcA within an infected cell, providing validation for previous in vitro findings. Induction of chlamydial heat shock gene expression by elevated temperature was due to loss of HrcA binding to heat shock promoters, supporting a mechanism of derepression. This heat shock response was rapid, whereas recovery of HrcA binding and return to non-stress transcript levels occurred more slowly. We also found that control of heat shock gene expression was differentially regulated over the course of the intracellular Chlamydia infection. There was evidence of HrcA-mediated regulation of heat shock genes throughout the chlamydial developmental cycle, but the level of repression was lower at early times. This is the first study of Chlamydia-infected cells showing the effect of an environmental signal on transcription factor-DNA binding and target gene expression in the bacterium.
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