Evidence map›Paper›PMID 26075609›Full record

ArticlePloS one2015

Estradiol and GPER Activation Differentially Affect Cell Proliferation but Not GPER Expression in the Hippocampus of Adult Female Rats.

Paula Duarte-Guterman, Stephanie E Lieblich, Carmen Chow, Liisa A M Galea

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
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  6. Perinatal activation of ERJournal of neurophysiology · 2021
    Article
  7. Review
  8. Review
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  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
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  18. Review
  19. Why estrogens matter for behavior and brain health.Neuroscience and biobehavioral reviews · 2017
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Paula Duarte-GutermanDepartment of Psychology, Program in Neuroscience, Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
Stephanie E LieblichDepartment of Psychology, Program in Neuroscience, Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
Carmen ChowDepartment of Psychology, Program in Neuroscience, Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
Liisa A M GaleaDepartment of Psychology, Program in Neuroscience, Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada.
University of British Columbia · CA

Funding

Canadian Institutes of Health Research MOP102568
6 · The paper itself

Abstract

Estradiol increases cell proliferation in the dentate gyrus of the female rodent but it is not known whether the G protein-coupled estrogen receptor (GPER), a membrane receptor, is involved in this process, nor whether there are regional differences in estradiol's effects on cell proliferation. Thus, we investigated whether estradiol exerts its effects on cell proliferation in the dorsal and ventral dentate gyrus through GPER, using the GPER agonist, G1, and antagonist, G15. Ovariectomized adult female rats received a single injection of either: 17β-estradiol (10 μg), G1 (0.1, 5, 10 μg), G15 (40 μg), G15 and estradiol, or vehicle (oil, DMSO, or oil+DMSO). After 30 min, animals received an injection of bromodeoxyuridine (BrdU) and were perfused 24 h later. Acute treatment with estradiol increased, while the GPER agonist G1 (5 μg) decreased, the number of BrdU+ cells in the dentate gyrus relative to controls. The GPER antagonist, G15 increased the number of BrdU+ cells relative to control in the dorsal region and decreased the number of BrdU+ cells in the ventral region. However, G15 treatment in conjunction with estradiol partially eliminated the estradiol-induced increase in cell proliferation in the dorsal dentate gyrus. Furthermore, G1 decreased the expression of GPER in the dentate gyrus but not the CA1 and CA3 regions of the hippocampus. In summary, we found that activation of GPER decreased cell proliferation and GPER expression in the dentate gyrus of young female rats, presenting a potential and novel estrogen-independent role for this receptor in the adult hippocampus.

Indexed as

AnimalsBenzodioxolesBody WeightCell ProliferationCyclopentanesDentate GyrusEstradiolFemaleGene Expression RegulationHippocampusKi-67 AntigenOrgan SizeQuinolinesRatsReceptors, G-Protein-Coupled1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinolineBenzodioxolesCyclopentanesEstradiolGper1 protein, ratKi-67 AntigenQuinolinesReceptors, G-Protein-Coupled

Identifiers

PMID26075609
PMCPMC4468121
OpenAlexW609159563

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.