Evidence map›Paper›PMID 26075520›Full record

ArticleNature structural & molecular biology2015

The Anopheles-midgut APN1 structure reveals a new malaria transmission-blocking vaccine epitope.

Sarah C Atkinson, Jennifer S Armistead, Derrick K Mathias, Maurice M Sandeu, Dingyin Tao, Nahid Borhani-Dizaji, Brian B Tarimo, Isabelle Morlais, Rhoel R Dinglasan, Natalie A Borg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
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  7. Article
  8. Article
  9. Monoclonal antibodies for malaria prevention.Molecular therapy : the journal of the American Society of Gene Therapy · 2022
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  10. Review
  11. Article
  12. Article
  13. Targeting Gametocytes of the Malaria ParasitePathogens (Basel, Switzerland) · 2021
    Review
  14. Progress towards the development of aExpert review of vaccines · 2021
    Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. A Hetero-Multimeric Chitinase-ContainingFrontiers in cellular and infection microbiology · 2020
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Sarah C AtkinsonDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Jennifer S Armistead1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Derrick K Mathias1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Maurice M SandeuLaboratoire de Recherche sur le Paludisme, Institut de Recherche pour le Développement-Organisation de Coordination et de Coopération pour la Lutte Contre les Grandes Endémies en Afrique Centrale, Yaoundé, Cameroon.
Dingyin Tao1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Nahid Borhani-Dizaji1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Brian B Tarimo1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [3] Nelson Mandela African Institute for Science and Technology, Arusha, Tanzania. [4] Ifakara Health Institute, Dar es Salaam, Tanzania.
Isabelle MorlaisLaboratoire de Recherche sur le Paludisme, Institut de Recherche pour le Développement-Organisation de Coordination et de Coopération pour la Lutte Contre les Grandes Endémies en Afrique Centrale, Yaoundé, Cameroon.
Rhoel R Dinglasan1] W. Harry Feinstone Department of Molecular Microbiology &Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA. [2] Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Natalie A BorgDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Johns Hopkins University · USMonash University · AUOrganisation de Coordination pour la lutte contre les Endémies en Afrique Centrale · CM

Funding

Institute for Clinical and Translational Research (UL1)UL1RR025005 · NCRR · JOHNS HOPKINS UNIVERSITY · PI FORD, DANIEL ERNEST · 2007 to 2011
$75.8M
ZINC AND CA EDTA THERAPY OF PLUMBISMM01RR000052 · NCRR · JOHNS HOPKINS UNIVERSITY · PI BELCHER, HAROLYN MILLICENT EDITH · 1985 to 2007
$37.3M
NCRR NIH HHS M01 RR000052NCRR NIH HHS UL1 RR025005
6 · The paper itself

Abstract

Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antibodies functionally block parasite transmission have remained elusive. Here we present the 2.65-Å crystal structure of AnAPN1 and the immunoreactivity and transmission-blocking profiles of three monoclonal antibodies (mAbs) to AnAPN1, including mAb 4H5B7, which effectively blocks transmission of natural strains of Plasmodium falciparum. Using the AnAPN1 structure, we map the conformation-dependent 4H5B7 neoepitope to a previously uncharacterized region on domain 1 and further demonstrate that nonhuman-primate neoepitope-specific IgG also blocks parasite transmission. We discuss the prospect of a new biological function of AnAPN1 as a receptor for Plasmodium in the mosquito midgut and the implications for redesigning the AnAPN1 mTBV.

Indexed as

AnimalsAnophelesAntibodies, MonoclonalCatalytic DomainCD13 AntigensCrystallography, X-RayEpitope MappingHumansMalariaMalaria VaccinesModels, MolecularPlasmodium falciparumProtein ConformationProtein MultimerizationAntibodies, MonoclonalCD13 AntigensMalaria Vaccines

Identifiers

PMID26075520
PMCPMC4547048
OpenAlexW1536557355

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.