ArticleNature cell biology2015
Reconstitution of mitotic chromatids with a minimum set of purified factors.
Article in Nature cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed, 181 citations in OpenAlex.
- Dual phosphoregulatory mechanisms of condensin I revealed by biochemical reconstitution.PNAS nexus · 2026Article
- Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Safeguarding genome integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 orchestrate Topoisomerase II-mediated catenane resolution in mitosis.Nucleic acids research · 2026Article
- Setting Up to Divide: Nuclear Mechanotransduction in the Regulation of Mitosis.Results and problems in cell differentiation · 2026Review
- Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026Review
- Functional interplay between condensin I and topoisomerase Iiα in single-molecule DNA compaction.Nature communications · 2025Article
- Calyculin A Induces Premature Chromosome Condensation and Chromatin Compaction in GbioRxiv : the preprint server for biology · 2025Article
- Mitotic genome folding.The Journal of cell biology · 2025Review
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- SRBD1 facilitates chromosome segregation by promoting topoisomerase IIα localization to mitotic chromosomes.Nature communications · 2025Article
- Review
- NCAPD3 promotes diffuse large B-cell lymphoma progression through modulating SIRT1 expression in an H3K9 monomethylation-dependent manner.Journal of advanced research · 2025Article
- Structural Maintenance of Chromosomes Complexes.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Review
- Multiwell-based G0-PCC assay for radiation biodosimetry.Scientific reports · 2024Article
- Regulation of condensin II by self-suppression and release mechanisms.Molecular biology of the cell · 2024Article
- Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro.PloS one · 2024Article
- Article
- Elasticity control of entangled chromosomes: Crosstalk between condensin complexes and nucleosomes.Biophysical journal · 2023Article
- The material properties of mitotic chromosomes.Current opinion in structural biology · 2023Review
44 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of mitotic chromosomes, each composed of a pair of rod-shaped chromatids, is an essential prerequisite for accurate transmission of the genome during cell division. It remains poorly understood, however, how this fundamental process might be achieved and regulated in the cell. Here we report an in vitro system in which mitotic chromatids can be reconstituted by mixing a simple substrate with only six purified factors: core histones, three histone chaperones (nucleoplasmin, Nap1 and FACT), topoisomerase II (topo II) and condensin I. We find that octameric nucleosomes containing the embryonic variant H2A.X-F are highly susceptible to FACT and function as the most productive substrate for subsequent actions of topo II and condensin I. Cdk1 phosphorylation of condensin I is the sole mitosis-specific modification required for chromatid reconstitution. This experimental system will enhance our understanding of the mechanisms of action of individual factors and their cooperation during this process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.