ArticleFrontiers in plant science2015
Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Cell-type-specific compartmentalization and function of the glucosinolate-myrosinase system in Arabidopsis thaliana.The Journal of biological chemistry · 2026Article
- Identification of key regulators for the development of Prunus leaves using single-cell RNA sequencing.BMC plant biology · 2026Article
- Proximity Labeling in Plants.Annual review of plant biology · 2023Review
- Arabidopsis Ubiquitin-Conjugating Enzymes UBC4, UBC5, and UBC6 Have Major Functions in Sugar Metabolism and Leaf Senescence.International journal of molecular sciences · 2022Article
- Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells.Plant physiology · 2022Article
- Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV.Nucleic acids research · 2021Article
- An Argon-Ion-Induced Pale Green Mutant ofPlants (Basel, Switzerland) · 2021Article
- GABA signalling modulates stomatal opening to enhance plant water use efficiency and drought resilience.Nature communications · 2021Article
- InFrontiers in plant science · 2020Article
- Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis.Journal of experimental botany · 2020Article
- Targeting intracellular transport combined with efficient uptake and storage significantly increases grain iron and zinc levels in rice.Plant biotechnology journal · 2019Article
- Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers.Frontiers in plant science · 2019Article
- Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation.Journal of visualized experiments : JoVE · 2018Article
- Global analysis of ribosome-associated noncoding RNAs unveils new modes of translational regulation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Decipher the Molecular Response of Plant Single Cell Types to Environmental Stresses.BioMed research international · 2016Review
- Meselect - A Rapid and Effective Method for the Separation of the Main Leaf Tissue Types.Frontiers in plant science · 2016Article
- Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots.Horticulture research · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Protein and transcript levels are partly decoupled as a function of translation efficiency and protein degradation. Selective protein degradation via the Ubiquitin-26S proteasome system (UPS) ensures protein homeostasis and facilitates adjustment of protein abundance during changing environmental conditions. Since individual leaf tissues have specialized functions, their protein composition is different and hence also protein level regulation is expected to differ. To understand UPS function in a tissue-specific context we developed a method termed Meselect to effectively and rapidly separate Arabidopsis thaliana leaf epidermal, vascular and mesophyll tissues. Epidermal and vascular tissue cells are separated mechanically, while mesophyll cells are obtained after rapid protoplasting. The high yield of proteins was sufficient for tissue-specific proteome analyses after inhibition of the proteasome with the specific inhibitor Syringolin A (SylA) and affinity enrichment of ubiquitylated proteins. SylA treatment of leaves resulted in the accumulation of 225 proteins and identification of 519 ubiquitylated proteins. Proteins that were exclusively identified in the three different tissue types are consistent with specific cellular functions. Mesophyll cell proteins were enriched for plastid membrane translocation complexes as targets of the UPS. Epidermis enzymes of the TCA cycle and cell wall biosynthesis specifically accumulated after proteasome inhibition, and in the vascular tissue several enzymes involved in glucosinolate biosynthesis were found to be ubiquitylated. Our results demonstrate that protein level changes and UPS protein targets are characteristic of the individual leaf tissues and that the proteasome is relevant for tissue-specific functions.
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