ArticleFrontiers in immunology2015
A Systematic Approach to Identify Markers of Distinctly Activated Human Macrophages.
Article in Frontiers in immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Equine monocyte-derived macrophages revisited: isolation and comprehensive characterization of pro- versus anti-inflammatory polarisation.The veterinary quarterly · 2025Article
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- Regulatory macrophages in solid organ xenotransplantation.Korean journal of transplantation · 2023Review
- IL-4 and IL-13 induce equivalent expression of traditional M2 markers and modulation of reactive oxygen species in human macrophages.Scientific reports · 2023Article
- Macrophage Biology in Human Granulomatous Skin Inflammation.International journal of molecular sciences · 2023Review
- Use of integrated metabolomics, transcriptomics, and signal protein profile to characterize the effector function and associated metabotype of polarized macrophage phenotypes.Journal of leukocyte biology · 2022Article
- Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer.BMC cancer · 2022Article
- Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer.Journal for immunotherapy of cancer · 2020Article
- Intra-articular depletion of macrophages increases acute synovitis and alters macrophage polarity in the injured mouse knee.Osteoarthritis and cartilage · 2020Article
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- Metabolic immunomodulation of macrophage functional plasticity in nonhealing wounds.Current opinion in infectious diseases · 2019Review
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- Macrophage nuclear receptors: Emerging key players in infectious diseases.PLoS pathogens · 2019Review
- Distinct Redox Signalling following Macrophage Activation Influences Profibrotic Activity.Journal of immunology research · 2019Article
- Metabolic Programming of Macrophages: Implications in the Pathogenesis of Granulomatous Disease.Frontiers in immunology · 2019Review
- Transcriptome Analysis Provides Insights into the Markers of Resting and LPS-Activated Macrophages in Grass Carp (International journal of molecular sciences · 2018Article
- MicroRNA networks associated with active systemic juvenile idiopathic arthritis regulate CD163 expression and anti-inflammatory functions in macrophages through two distinct mechanisms.Journal of leukocyte biology · 2018Article
- Differential Activation of Human Keratinocytes by Leishmania Species Causing Localized or Disseminated Disease.The Journal of investigative dermatology · 2017Article
- Species dependent impact of helminth-derived antigens on human macrophages infected with Mycobacterium tuberculosis: Direct effect on the innate anti-mycobacterial response.PLoS neglected tropical diseases · 2017Article
- Direct in vivo evidence of activated macrophages in human osteoarthritis.Osteoarthritis and cartilage · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Polarization has been a useful concept for describing activated macrophage phenotypes and gene expression profiles. However, macrophage activation status within tumors and other settings are often inferred based on only a few markers. Complicating matters for relevance to human biology, many macrophage activation markers have been best characterized in mice and sometimes are not similarly regulated in human macrophages. To identify novel markers of activated human macrophages, gene expression profiles for human macrophages of a single donor subjected to 33 distinct activating conditions were obtained and a set of putative activation markers were subsequently evaluated in macrophages from multiple donors using integrated fluidic circuit (IFC)-based RT-PCR. Using unsupervised hierarchical clustering of the microarray screen, highly altered transcripts (>4-fold change in expression) sorted the macrophage transcription profiles into two major and 13 minor clusters. Among the 1874 highly altered transcripts, over 100 were uniquely altered in one major or two related minor clusters. IFC PCR-derived data confirmed the microarray results and determined the kinetics of expression of potential macrophage activation markers. Transcripts encoding chemokines, cytokines, and cell surface were prominent in our analyses. The activation markers identified by this study could be used to better characterize tumor-associated macrophages from biopsies as well as other macrophage populations collected from human clinical samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.