ArticleFrontiers in microbiology2015
Anoxic growth of Ensifer meliloti 1021 by N2O-reduction, a potential mitigation strategy.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- A novelApplied and environmental microbiology · 2026Article
- Respiration and growth ofMicrobiology spectrum · 2024Article
- Sustained bacterial NNature communications · 2024Article
- Bradyrhizobium ontarionense sp. nov., a novel bacterial symbiont isolated from Aeschynomene indica (Indian jointvetch), harbours photosynthesis, nitrogen fixation and nitrous oxide (NAntonie van Leeuwenhoek · 2024Article
- Bacterial denitrification drives elevated NScience advances · 2023Article
- Bradyrhizobium ottawaense efficiently reduces nitrous oxide through high nosZ gene expression.Scientific reports · 2023Article
- Regulation of the Emissions of the Greenhouse Gas Nitrous Oxide by the Soybean EndosymbiontInternational journal of molecular sciences · 2022Article
- Improving Nitrogen Use Efficiency in Aerobic Rice Based on Insights Into the Ecophysiology of Archaeal and Bacterial Ammonia Oxidizers.Frontiers in plant science · 2022Review
- Recent trends in nitrogen cycle and eco-efficient nitrogen management strategies in aerobic rice system.Frontiers in plant science · 2022Review
- The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-NMicrobial ecology · 2020Article
- Host Range and Symbiotic Effectiveness of NFrontiers in microbiology · 2019Article
- Nitrogen Cycling in Soybean Rhizosphere: Sources and Sinks of Nitrous Oxide (NFrontiers in microbiology · 2019Review
- Absence of the Nitrous Oxide Reductase Gene Cluster in Commercial Alfalfa Inoculants Is Probably Due to the Extensive Loss of Genes During Rhizobial Domestication.Microbial ecology · 2018Article
- Biokinetic Characterization and Activities of NFrontiers in microbiology · 2018Article
- Rapid Succession of Actively Transcribing Denitrifier Populations in Agricultural Soil During an Anoxic Spell.Frontiers in microbiology · 2018Article
- Soil biochar amendment affects the diversity of nosZ transcripts: Implications for NScientific reports · 2017Article
- Gas entrapment and microbial NScientific reports · 2016Article
- Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Denitrification in agricultural soils is a major source of N2O. Legume crops enhance N2O emission by providing N-rich residues, thereby stimulating denitrification, both by free-living denitrifying bacteria and by the symbiont (rhizobium) within the nodules. However, there are limited data concerning N2O production and consumption by endosymbiotic bacteria associated with legume crops. It has been reported that the alfalfa endosymbiont Ensifer meliloti strain 1021, despite possessing and expressing the complete set of denitrification enzymes, is unable to grow via nitrate respiration under anoxic conditions. In the present study, we have demonstrated by using a robotized incubation system that this bacterium is able to grow through anaerobic respiration of N2O to N2. N2O reductase (N2OR) activity was not dependent on the presence of nitrogen oxyanions or NO, thus the expression could be induced by oxygen depletion alone. When incubated at pH 6, E. meliloti was unable to reduce N2O, corroborating previous observations found in both, extracted soil bacteria and Paracoccus denitrificans pure cultures, where expression of functional N2O reductase is difficult at low pH. Furthermore, the presence in the medium of highly reduced C-substrates, such as butyrate, negatively affected N2OR activity. The emission of N2O from soils can be lowered if legumes plants are inoculated with rhizobial strains overexpressing N2O reductase. This study demonstrates that strains like E. meliloti 1021, which do not produce N2O but are able to reduce the N2O emitted by other organisms, could act as even better N2O sinks.
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