ArticleFrontiers in microbiology2015
Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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16 citing papers in PubMed.
- Antibody-based sensing of pesticide residues and mycotoxins in plant-derived foods: A narrative review of ELISA, lateral-flow immunoassays and immunosensors.Food chemistry: X · 2026Review
- Comparative design of artificial ochratoxin A antigens: conjugation strategies and immunogenicity evaluation for antibody production.Frontiers in veterinary science · 2026Article
- Development of an immunoassay for the detection of mycotoxins using xMAP technology and its evaluation in black tea samples.Journal of food science and technology · 2024Article
- Pixel-Based Machine Learning and Image Reconstitution for Dot-ELISA Pathogen Diagnosis in Biological Samples.Frontiers in microbiology · 2021Article
- A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection.Foods (Basel, Switzerland) · 2020Article
- Inhibitory effect ofBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay.Frontiers in veterinary science · 2020Article
- Development of ELISA and Lateral Flow Immunoassays for Ochratoxins (OTA and OTB) Detection Based on Monoclonal Antibody.Frontiers in cellular and infection microbiology · 2020Article
- Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection.Frontiers in chemistry · 2020Review
- Detection and functional characterization of sigma class GST in Phlebotomus argentipes and its role in stress tolerance and DDT resistance.Scientific reports · 2019Article
- Novel haptens and monoclonal antibodies with subnanomolar affinity for a classical analytical target, ochratoxin A.Scientific reports · 2018Article
- Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its Evaluation Onto Various Field Samples.Frontiers in pharmacology · 2018Article
- Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay.Toxins · 2017Article
- Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1.Frontiers in microbiology · 2016Article
- Comparison of Fluorescent Microspheres and Colloidal Gold as Labels in Lateral Flow Immunochromatographic Assays for the Detection of T-2 Toxin.Molecules (Basel, Switzerland) · 2015Article
- A Novel Nano Magnetic Beads Dot ELISA Immunoassay and Its Application on the Detection ofIranian journal of parasitologyArticle
Corrections and comments
- Erratum issued
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In the present study, generation and characterization of a highly specific monoclonal antibody (mAb) against Ochratoxin A (OTA) was undertaken. The generated mAb was further used to develop a simple, fast, and sensitive sandwich dot-ELISA (s-dot ELISA) method for detection of OTA from contaminated food grain samples. The limit of detection (LOD) of the developed enzyme-linked immunosorbent assay (ELISA) method was determined as 5.0 ng/mL of OTA. Developed method was more specific toward OTA and no cross reactivity was observed with the other tested mycotoxins such as deoxynivalenol, fumonisin B1, or aflatoxin B1. To assess the utility and reliability of the developed method, several field samples of maize, wheat and rice (n = 195) collected from different geographical regions of southern Karnataka region of India were evaluated for the OTA occurrence. Seventy two out of 195 samples (19 maize, 38 wheat, and 15 rice) were found to be contaminated by OTA by s-dot ELISA. The assay results were further co-evaluated with conventional analytical high-performance liquid chromatography (HPLC) method. Results of the s-dot ELISA are in concordance with HPLC except for three samples that were negative for OTA presence by s-dot ELISA but found positive by HPLC. Although positive by HPLC, the amount of OTA in the three samples was found to be lesser than the accepted levels (>5 μg/kg) of OTA presence in cereals. Therefore, in conclusion, the developed s-dot ELISA is a better alternative for routine cereal based food and feed analysis in diagnostic labs to check the presence of OTA over existing conventional culture based, tedious analytical methods.
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