ArticleWorld neurosurgery2015
Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.
Article in World neurosurgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Role of MEF2C in the Endothelial Cells Derived from Human Induced Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2023Article
- Transcriptional reprogramming restores UBE3A brain-wide and rescues behavioral phenotypes in an Angelman syndrome mouse model.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Postmortem Human Dura Mater Cells Exhibit Phenotypic, Transcriptomic and Genetic Abnormalities that Impact their Use for Disease Modeling.Stem cell reviews and reports · 2022Article
- HDACs regulate the differentiation of endothelial cells from human iPSCs.Cell biochemistry and function · 2022Article
- Applying hiPSCs and Biomaterials Towards an Understanding and Treatment of Traumatic Brain Injury.Frontiers in cellular neuroscience · 2020Review
- Using miniature brain implants in rodents for novel drug discovery.Expert opinion on drug discovery · 2019Review
- Advance of Stem Cell Treatment for Traumatic Brain Injury.Frontiers in cellular neuroscience · 2019Review
- Generation of human vascularized brain organoids.Neuroreport · 2018Article
- Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome.Stem cell research & therapy · 2017Article
- Induced Pluripotent Stem Cells: Generation Strategy and Epigenetic Mystery behind Reprogramming.Stem cells international · 2016Review
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10 authors at 2 institutions in 1 country.
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Abstract
backgroundThe dura mater can be easily biopsied during most cranial neurosurgical operations. We describe a protocol that allows for robust generation of induced pluripotent stem cells (iPSCs) and neural progenitors from acutely harvested dura mater.
objectiveTo generate iPSCs and neural progenitor cells from dura mater obtained during ventriculoperitoneal shunt surgery.
methodsDura was obtained during ventriculoperitoneal shunt surgery for normal pressure hydrocephalus from a 60-year-old patient with severe cognitive impairment. Fibroblasts were isolated from the dural matrix and transduced with nonintegrating Sendai virus for iPSC induction. A subset of successfully generated iPSC clones underwent immunocytochemical analysis, teratoma assay, karyotyping, and targeted neural differentiation.
resultsEleven iPSC clones were obtained from the transduction of an estimated 600,000 dural fibroblasts after 3 passages. Three clones underwent immunocytochemical analysis and were shown to express the transcription factors OCT-4, SOX2, and the embryonic cell markers SSEA-4, TRA-1-60, and Nanog. Two clones were tested for pluripotency and formed teratomas at the injection site in immunodeficient mice. Three clones underwent chromosomal analysis and were found to have a normal metaphase spread and karyotype. One clone underwent targeted neural differentiation and formed neural rosettes as well as TuJ1/SOX1-positive neural progenitor cells.
conclusionsIPSCs and neural progenitor cells can be efficiently derived from the dura of patients who need to undergo cranial neurosurgical operations. IPSCs were obtained with a nonintegrating virus and exhibited a normal karyotype, making them candidates for future autotransplantation after targeted differentiation to treat functional deficits.
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