ArticleJournal of surgical oncology2015
A ratiometric threshold for determining presence of cancer during fluorescence-guided surgery.
Article in Journal of surgical oncology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Introducing fluorescence guided surgery into orthopedic oncology: A systematic review of candidate protein targets for Ewing sarcoma.Journal of surgical oncology · 2018Pooled it
- Fluorescence Imaging-Assessed Surgical Margin Detection in Head and Neck Oncology by Passive and Active Targeting.Molecular diagnosis & therapy · 2025Review
- Background Tissue with Native Target Expression Can Determine Presence of Nodal Metastasis in Head and Neck Squamous Cell Carcinoma Patients Infused with Targeted Fluorescent Tracers.Molecular imaging and biology · 2025Article
- Article
- Illuminating the future of precision cancer surgery with fluorescence imaging and artificial intelligence convergence.NPJ precision oncology · 2024Review
- Recent advances in ratiometric fluorescence imaging of enzyme activity in vivo.Current opinion in chemical biology · 2024Review
- Preclinical assessment of IRDye800CW-labeled gastrin-releasing peptide receptor-targeting peptide for near infrared-II imaging of brain malignancies.Bioengineering & translational medicine · 2023Article
- Fluorescently Labeled Cetuximab-IRDye800 for Guided Surgical Excision of Ameloblastoma: A Proof of Principle Study.Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons · 2020Article
- Potential Receptors for Targeted Imaging of Lymph Node Metastases in Penile Cancer.Diagnostics (Basel, Switzerland) · 2020Article
- Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications.Diagnostics (Basel, Switzerland) · 2020Review
- Near infrared readouts offer sensitive and rapid assessments of intestinal permeability and disease severity in inflammatory bowel disease models.Scientific reports · 2020Article
- In-vivo optical imaging in head and neck oncology: basic principles, clinical applications and future directions.International journal of oral science · 2018Review
- Specimen mapping in head and neck cancer using fluorescence imaging.Laryngoscope investigative otolaryngology · 2017Review
- Selection of optimal molecular targets for tumor-specific imaging in pancreatic ductal adenocarcinoma.Oncotarget · 2017Article
- Sensitivity and Specificity of Cetuximab-IRDye800CW to Identify Regional Metastatic Disease in Head and Neck Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2017Article
- Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients.Journal of biomedical optics · 2017Article
- Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2017Article
- Signal to Noise Ratio as a Cross-Platform Metric for Intraoperative Fluorescence Imaging.Molecular imagingArticle
- Tumor margin assessment of surgical tissue specimen of cancer patients using label-free hyperspectral imaging.Proceedings of SPIE--the International Society for Optical EngineeringArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
BACKGROUND AND
objectiveFluorescence-guided imaging to assist in identification of malignant margins has the potential to dramatically improve oncologic surgery. However, a standardized method for quantitative assessment of disease-specific fluorescence has not been investigated. Introduced here is a ratiometric threshold derived from mean fluorescent tissue intensity that can be used to semi-quantitatively delineate tumor from normal tissue.
methodsOpen-field and a closed-field imaging devices were used to quantify fluorescence in punch biopsy tissues sampled from primary tumors collected during a phase 1 trial evaluating the safety of cetuximab-IRDye800 in patients (n = 11) undergoing surgical intervention for head and neck cancer. Fluorescence ratios were calculated using mean fluorescence intensity (MFI) from punch biopsy normalized by MFI of patient-matched tissues. Ratios were compared to pathological assessment and a ratiometric threshold was established to predict presence of cancer.
resultsDuring open-field imaging using an intraoperative device, the threshold for muscle normalized tumor fluorescence was found to be 2.7, which produced a sensitivity of 90.5% and specificity of 78.6% for delineating disease tissue. The skin-normalized threshold generated greater sensitivity (92.9%) and specificity (81.0%).
conclusionSuccessful implementation of a semi-quantitative threshold can provide a scientific methodology for delineating disease from normal tissue during fluorescence-guided resection of cancer.
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