ArticleNature communications2015
Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Recoding by NNature communications · 2026Article
- RNAViruses · 2026Review
- Kinetics of programmed and spontaneous ribosome sliding along the mRNA.Nucleic acids research · 2024Article
- Modulation of translational decoding by mNature communications · 2023Article
- Sordarin bound eEF2 unlocks spontaneous forward and reverse translocation on CrPV IRES.Nucleic acids research · 2023Article
- Conformational dynamics of the RNA binding channel regulates loading and translocation of the DEAH-box helicase Prp43.Nucleic acids research · 2023Article
- Regulation of the DEAH/RHA helicase Prp43 by the G-patch factor Pfa1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hyper-swivel head domain motions are required for complete mRNA-tRNA translocation and ribosome resetting.Nucleic acids research · 2022Article
- Altered tRNA dynamics during translocation on slippery mRNA as determinant of spontaneous ribosome frameshifting.Nature communications · 2022Article
- The Structural Dynamics of Translation.Annual review of biochemistry · 2022Review
- Lateral gate dynamics of the bacterial translocon during cotranslational membrane protein insertion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High-Resolution DNA Dual-Rulers Reveal a New Intermediate State in Ribosomal Frameshifting.Chembiochem : a European journal of chemical biology · 2021Article
- Structural insights into mammalian mitochondrial translation elongation catalyzed by mtEFG1.The EMBO journal · 2020Article
- Mechanisms and biomedical implications of -1 programmed ribosome frameshifting on viral and bacterial mRNAs.FEBS letters · 2019Review
- EF-G-induced ribosome sliding along the noncoding mRNA.Science advances · 2019Article
- New Insights into Ribosome Structure and Function.Cold Spring Harbor perspectives in biology · 2019Review
- tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.Cell reports · 2018Article
- The 2018 correlative microscopy techniques roadmap.Journal of physics D: Applied physics · 2018Review
- Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots.Nucleic acids research · 2018Article
- Translation in Prokaryotes.Cold Spring Harbor perspectives in biology · 2018Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The coupled translocation of transfer RNA and messenger RNA through the ribosome entails large-scale structural rearrangements, including step-wise movements of the tRNAs. Recent structural work has visualized intermediates of translocation induced by elongation factor G (EF-G) with tRNAs trapped in chimeric states with respect to 30S and 50S ribosomal subunits. The functional role of the chimeric states is not known. Here we follow the formation of translocation intermediates by single-molecule fluorescence resonance energy transfer. Using EF-G mutants, a non-hydrolysable GTP analogue, and fusidic acid, we interfere with either translocation or EF-G release from the ribosome and identify several rapidly interconverting chimeric tRNA states on the reaction pathway. EF-G engagement prevents backward transitions early in translocation and increases the fraction of ribosomes that rapidly fluctuate between hybrid, chimeric and posttranslocation states. Thus, the engagement of EF-G alters the energetics of translocation towards a flat energy landscape, thereby promoting forward tRNA movement.
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