ArticleNucleic acids research2015
FDF-PAGE: a powerful technique revealing previously undetected small RNAs sequestered by complementary transcripts.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Article
- Purification and in vivo, cell-free, and in vitro characterization of CRISPR-Cas12a2.Methods in enzymology · 2025Article
- Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference.Microbiology and molecular biology reviews : MMBR · 2023Review
- Virus and Viroid-Derived Small RNAs as Modulators of Host Gene Expression: Molecular Insights Into Pathogenesis.Frontiers in microbiology · 2020Review
- Paternal easiRNAs regulate parental genome dosage in Arabidopsis.Nature genetics · 2018Article
- Small RNA-Omics for Plant Virus Identification, Virome Reconstruction, and Antiviral Defense Characterization.Frontiers in microbiology · 2018Review
- Efficient targeted DNA editing and replacement inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs.Nucleic acids research · 2017Article
- Virus-derived small RNAs: molecular footprints of host-pathogen interactions.Wiley interdisciplinary reviews. RNA · 2016Review
- Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.Genome research · 2016Article
- Synergistic infection of two viruses MCMV and SCMV increases the accumulations of both MCMV and MCMV-derived siRNAs in maize.Scientific reports · 2016Article
- Article
- The Tomato Spotted Wilt Virus Genome Is Processed Differentially in its Plant Host Arachis hypogaea and its Thrips Vector Frankliniella fusca.Frontiers in plant science · 2016Article
- Viral Small-RNA Analysis of Bombyx mori Larval Midgut during Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.Journal of virology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Small RNAs, between 18nt and 30nt in length, are a diverse class of non-coding RNAs that mediate a range of cellular processes, from gene regulation to pathogen defense. They guide ribonucleoprotein complexes to their target nucleic acids by Watson-Crick base pairing. We report here that current techniques for small RNA detection and library generation are biased by formation of RNA duplexes. To address this problem, we established FDF-PAGE (fully-denaturing formaldehyde polyacrylamide gel electrophoresis) to prevent annealing of sRNAs to their complement. By applying FDF-PAGE, we provide evidence that both strands of viral small RNA are present in near equimolar ratios, indicating that the predominant precursor is a long double-stranded RNA. Comparing non-denaturing conditions to FDF-PAGE uncovered extensive sequestration of miRNAs in model organisms and allowed us to identify candidate small RNAs under the control of competing endogenous RNAs (ceRNAs). By revealing the full repertoire of small RNAs, we can begin to create a better understanding of small RNA mediated interactions.
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