ArticleAnnals of botany2015
SlbHLH068 interacts with FER to regulate the iron-deficiency response in tomato.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 35 citations in OpenAlex.
- AlfalfaFrontiers in plant science · 2024Article
- Iron in the Symbiosis of Plants and Microorganisms.Plants (Basel, Switzerland) · 2023Review
- Loss of function of the bHLH transcription factor Nrd1 in tomato enhances resistance to Pseudomonas syringae.Plant physiology · 2022Article
- Iron uptake, signaling, and sensing in plants.Plant communications · 2022Review
- UNFERTILIZED EMBRYO SAC 12 phosphorylation plays a crucial role in conferring salt tolerance.Plant physiology · 2022Article
- Pepper bHLH transcription factorHorticulture research · 2022Article
- Transcriptome Analysis Revealed the Molecular Response Mechanism of Non-heading Chinese Cabbage to Iron Deficiency Stress.Frontiers in plant science · 2022Article
- Downregulation of Zn-transporters along with Fe and redox imbalance causes growth and photosynthetic disturbance in Zn-deficient tomato.Scientific reports · 2021Article
- FIT, a regulatory hub for iron deficiency and stress signaling in roots, and FIT-dependent and -independent gene signatures.Journal of experimental botany · 2020Review
- 'Candidatus Phytoplasma solani' interferes with the distribution and uptake of iron in tomato.BMC genomics · 2019Article
- The Transcriptional Control of Iron Homeostasis in Plants: A Tale of bHLH Transcription Factors?Frontiers in plant science · 2019Article
- Mapping and Characterization of theFrontiers in plant science · 2017Article
- The ethylene response factor AtERF4 negatively regulates the iron deficiency response in Arabidopsis thaliana.PloS one · 2017Article
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Authors and funding
7 authors at 1 institution in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsIron is an essential micronutrient for all organisms and its uptake, translocation, distribution and utilization are regulated in a complex manner in plants. FER, isolated from tomato (Solanum lycopersicum), was the first transcription factor involved in the iron homeostasis of higher plants to be identified. A FER defect in the T3238fer mutant drastically downregulates the expression of iron uptake genes, such as ferric-chelate reductase 1 (LeFRO1) and iron-regulated transporter 1 (LeIRT1); however, the molecular mechanism by which FER regulates genes downstream remains unknown. The aim of this work was therefore to identify the gene that interacts with FER to regulate the iron-deficiency response in tomato.
methodsThe homologue of the Arabidopsis Ib subgroup of the basic helix-loop-helix (bHLH) proteins, SlbHLH068, was identified by using the program BLASTP against the AtbHLH39 amino acid sequence in the tomato genome. The interaction between SlbHLH068 and FER was detected using yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. In addition, virus-induced gene silencing (VIGS) was used to generate tomato plants in which SlbHLH068 expression was downregulated. The expression of genes was analysed using northern blot hybridization and multiple RT-PCR analysis. Seedlings of wild-type and mutant plants were grown under conditions of different nutrient deficiency. KEY
resultsSlbHLH068 is highly upregulated in roots, leaves and stems in response to iron deficiency. An interaction between SlbHLH068 and FER was demonstrated using yeast two-hybrid and BiFC assays. The heterodimer formed by FER with SlbHLH068 directly bound to the promoter of LeFRO1 and activated the expression of its reporter gene in the yeast assay. The downregulation of SlbHLH068 expression by VIGS resulted in a reduction of LeFRO1 and LeIRT1 expression and iron accumulation in leaves and roots.
conclusionsThe results indicate that SlbHLH068, as a putative transcription factor, is involved in iron homeostasis in tomato via an interaction with FER.
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