ArticlePloS one2015
High-Throughput Sequencing and De Novo Assembly of Red and Green Forms of the Perilla frutescens var. crispa Transcriptome.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- CRISPR-Cas9 disruption of flavanone 3-hydroxylase produces a green phenotype and alters flavone metabolites in allotetraploid perilla.Frontiers in plant science · 2026Article
- Genome-wide analysis of PfBBXs transcription factors in Perilla frutescens and their expression responses to different light intensities.Plant molecular biology · 2025Article
- Metabolomic and Transcriptomic Analyses Reveal the Factors Underlying Mature Fruit Pericarp Color Variations in the 'Xinli No. 7' Pear (Metabolites · 2025Article
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Utilization of NovelPlants (Basel, Switzerland) · 2022Article
- Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update.Plants (Basel, Switzerland) · 2022Review
- Neuroinflammation: A Potential Risk for Dementia.International journal of molecular sciences · 2022Review
- Incipient diploidization of the medicinal plant Perilla within 10,000 years.Nature communications · 2021Article
- Analysis of genetic diversity and relationships of Perilla frutescens using novel EST-SSR markers derived from transcriptome between wild-type and mutant Perilla.Molecular biology reports · 2021Article
- Multi-omics analysis of the bioactive constituents biosynthesis of glandular trichome in Perilla frutescens.BMC plant biology · 2021Article
- Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens (L.) leaves.Scientific reports · 2020Article
- Biochemical Characterization of a FlavonoidMolecules (Basel, Switzerland) · 2020Article
- Transcriptome analysis of Clinopodium gracile (Benth.) Matsum and identification of genes related to Triterpenoid Saponin biosynthesis.BMC genomics · 2020Article
- Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence.Scientific reports · 2019Article
- Drying the leaves ofFood science & nutrition · 2019Article
- Genetic diversity and population structure among accessions of Perilla frutescens (L.) Britton in East Asia using new developed microsatellite markers.Genes & genomics · 2018Article
- Third DWF1 paralog in Solanaceae, sterol ΔProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Transcriptomic analysis of Perilla frutescens seed to insight into the biosynthesis and metabolic of unsaturated fatty acids.BMC genomics · 2018Article
- Transcription Factor SsSte12 Was Involved in Mycelium Growth and Development inFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Perilla frutescens var. crispa (Labiatae) has two chemo-varietal forms, i.e. red and green forms of perilla, that differ in the production of anthocyanins. To facilitate molecular biological and biochemical studies in perilla-specialized metabolism we used Illumina RNA-sequencing technology in our comprehensive comparison of the transcriptome map of the leaves of red and green forms of perilla. Sequencing generated over 1.2 billion short reads with an average length of 101 nt. De novo transcriptome assembly yielded 47,788 and 47,840 unigenes in the red and green forms of perilla plants, respectively. Comparison of the assembled unigenes and existing perilla cDNA sequences showed highly reliable alignment. All unigenes were annotated with gene ontology (GO) and Enzyme Commission numbers and entered into the Kyoto Encyclopedia of Genes and Genomes. We identified 68 differentially expressed genes (DEGs) in red and green forms of perilla. GO enrichment analysis of the DEGs showed that genes involved in the anthocyanin metabolic process were enriched. Differential expression analysis revealed that the transcript level of anthocyanin biosynthetic unigenes encoding flavonoid 3'-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase was significantly higher in red perilla, while the transcript level of unigenes encoding limonene synthase was significantly higher in green perilla. Our data serve as a basis for future research on perilla bio-engineering and provide a shortcut for the characterization of new functional genes in P. frutescens.
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