ArticlePloS one2015
Disruption of bbe02 by Insertion of a Luciferase Gene Increases Transformation Efficiency of Borrelia burgdorferi and Allows Live Imaging in Lyme Disease Susceptible C3H Mice.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Comparative analysis of Borrelia's Defence mechanisms and their impact on genetic manipulation of low-passage isolates ofCurrent research in microbial sciences · 2026Article
- Hypercholesterolemia enhances early dissemination and Borrelia burgdorferi burden in a mouse model.Microbial pathogenesis · 2025Article
- Long-term survival ofMicrobiology spectrum · 2025Article
- Temporal Dynamic Interplay of Mouse Proteome during ProtozoanJournal of proteome research · 2025Article
- Article
- Analysis of bb0556 Expression and Its Role During Borrelia burgdorferi Mammalian Infection.Molecular microbiology · 2024Article
- Optimising Transformation Efficiency inInternational journal of molecular sciences · 2024Article
- Hitchhiker's Guide toJournal of bacteriology · 2024Review
- SCGB1D2 inhibits growth of Borrelia burgdorferi and affects susceptibility to Lyme disease.Nature communications · 2024Article
- Role of the Hypothetical Protein BB0563 during Borrelia burgdorferi Infection in Animals.Infection and immunity · 2023Article
- Sciatic-Vagal Nerve Stimulation by Electroacupuncture Alleviates Inflammatory Arthritis in Lyme Disease-Susceptible C3H Mice.Frontiers in immunology · 2022Article
- Pathogenesis of Borrelia burgdorferi and Babesia microti in TLR4-Competent and TLR4-dysfunctional C3H mice.Cellular microbiology · 2021Article
- Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Live Imaging.Current issues in molecular biology · 2021Review
- Evaluation of Nucleoside Analogs as Antimicrobials Targeting Unique Enzymes inPathogens (Basel, Switzerland) · 2020Article
- Effects of topical corticosteroids and lidocaine on Borrelia burgdorferi sensu lato in mouse skin: potential impact to human clinical trials.Scientific reports · 2020Article
- Protozoan ParasiteFrontiers in microbiology · 2019Article
- Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi.Applied and environmental microbiology · 2018Article
- The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization.Infection and immunity · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Lyme disease is the most prevalent tick-borne disease in North America and Europe. The causative agent, Borrelia burgdorferi persists in the white-footed mouse. Infection with B. burgdorferi can cause acute to persistent multisystemic Lyme disease in humans. Some disease manifestations are also exhibited in the mouse model of Lyme disease. Genetic manipulation of B. burgdorferi remains difficult. First, B. burgdorferi contains a large number of endogenous plasmids with unique sequences encoding unknown functions. The presence of these plasmids needs to be confirmed after each genetic manipulation. Second, the restriction modification defense systems, including that encoded by bbe02 gene lead to low transformation efficiency in B. burgdorferi. Therefore, studying the molecular basis of Lyme pathogenesis is a challenge. Furthermore, investigation of the role of a specific B. burgdorferi protein throughout infection requires a large number of mice, making it labor intensive and expensive. To overcome the problems associated with low transformation efficiency and to reduce the number of mice needed for experiments, we disrupted the bbe02 gene of a highly infectious and pathogenic B. burgdorferi strain, N40 D10/E9 through insertion of a firefly luciferase gene. The bbe02 mutant shows higher transformation efficiency and maintains luciferase activity throughout infection as detected by live imaging of mice. Infectivity and pathogenesis of this mutant were comparable to the wild-type N40 strain. This mutant will serve as an ideal parental strain to examine the roles of various B. burgdorferi proteins in Lyme pathogenesis in the mouse model in the future.
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