Evidence map›Paper›PMID 26069703›Full record

ArticleCartilage2014

Extensively Expanded Auricular Chondrocytes Form Neocartilage In Vivo.

Alan Tseng, Irina Pomerantseva, Michael J Cronce, Anya M Kimura, Craig M Neville, Mark A Randolph, Joseph P Vacanti, Cathryn A Sundback

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Auricular reconstruction: where are we now? A critical literature review.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2022
    Review
  8. Article
  9. Article
  10. Article
  11. Article
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  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Alan TsengDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA.
Irina PomerantsevaDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA ; Harvard Medical School, Boston, MA, USA.
Michael J CronceDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA.
Anya M KimuraDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA.
Craig M NevilleDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA ; Harvard Medical School, Boston, MA, USA.
Mark A RandolphHarvard Medical School, Boston, MA, USA ; Plastic Surgery Research Laboratory, Massachusetts General Hospital, Boston, MA, USA.
Joseph P VacantiDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA ; Harvard Medical School, Boston, MA, USA.
Cathryn A SundbackDepartment of Surgery, Massachusetts General Hospital, Boston, MA, USA ; Center for Regenerative Medicine, Boston, MA, USA ; Harvard Medical School, Boston, MA, USA.
Massachusetts General Hospital · USHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveOur goal was to engineer cartilage in vivo using auricular chondrocytes that underwent clinically relevant expansion and using methodologies that could be easily translated into health care practice.

designSheep and human chondrocytes were isolated from auricular cartilage biopsies and expanded in vitro. To reverse dedifferentiation, expanded cells were either mixed with cryopreserved P0 chondrocytes at the time of seeding onto porous collagen scaffolds or proliferated with basic fibroblast growth factor (bFGF). After 2-week in vitro incubation, seeded scaffolds were implanted subcutaneously in nude mice for 6 weeks. The neocartilage quality was evaluated histologically; DNA and glycosaminoglycans were quantified. Cell proliferation rates and collagen gene expression profiles were assessed.

resultsClinically sufficient over 500-fold chondrocyte expansion was achieved at passage 3 (P3); cell dedifferentiation was confirmed by the simultaneous COL1A1/3A1 gene upregulation and COL2A1 downregulation. The chondrogenic phenotype of sheep but not human P3 cells was rescued by addition of cryopreserved P0 chondrocytes. With bFGF supplementation, chondrocytes achieved clinically sufficient expansion at P2; COL2A1 expression was not rescued but COL1A1/3A1genes were downregulated. Although bFGF failed to rescue COL2A1 expression during chondrocyte expansion in vitro, elastic neocartilage with obvious collagen II expression was observed on porous collagen scaffolds after implantation in mice for 6 weeks.

conclusionsBoth animal and human auricular chondrocytes expanded with low-concentration bFGF supplementation formed high-quality elastic neocartilage on porous collagen scaffolds in vivo.

Indexed as

chondrocytesclinical-scale cell expansiondedifferentiationengineered cartilage

Identifiers

PMID26069703
PMCPMC4335768
OpenAlexW1988780490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.