ArticleRedox biology2015
Effects of long-term dietary nitrate supplementation in mice.
Article in Redox biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
27 citing papers in PubMed, 68 citations in OpenAlex.
- The Oral Microbiome-Nitrate-Nitrite-Nitric Oxide Axis and Cardiovascular Health: A Narrative Review.Journal of clinical medicine · 2026Review
- Salivary-Gland-Mediated Nitrate Recirculation as a Modulator for Cardiovascular Diseases.Biomolecules · 2025Review
- Increased Anion Exchanger-1 (Band 3) on the Red Blood Cell Membrane Accelerates Scavenging of Nitric Oxide Metabolites and Predisposes Hypertension Risks.Function (Oxford, England) · 2025Article
- Review
- Chronic inorganic nitrate supplementation does not improve metabolic health and worsens disease progression in mice with diet-induced obesity.American journal of physiology. Endocrinology and metabolism · 2025Article
- The Health Effects of Dietary Nitrate on Sarcopenia Development: Prospective Evidence from the UK Biobank.Foods (Basel, Switzerland) · 2024Article
- Exploring the wound healing potential of dietary nitrate in diabetic rat model.Frontiers in physiology · 2024Article
- From nitrate to NO: potential effects of nitrate-reducing bacteria on systemic health and disease.European journal of medical research · 2023Review
- Protective effects of long-term nitrate administration against ovariectomy-induced kidney dysfunction in rats.Pharmacological reports : PR · 2023Article
- Modulation of Insulin Resistance, Dyslipidemia and Serum Metabolome in iNOS Knockout Mice following Treatment with Nitrite, Metformin, Pioglitazone, and a Combination of Ampicillin and Neomycin.International journal of molecular sciences · 2021Article
- Article
- Dietary nitrate and population health: a narrative review of the translational potential of existing laboratory studies.BMC sports science, medicine & rehabilitation · 2021Review
- Systemic Insulin Resistance and Metabolic Perturbations in Chow Fed Inducible Nitric Oxide Synthase Knockout Male Mice: Partial Reversal by Nitrite Supplementation.Antioxidants (Basel, Switzerland) · 2020Article
- Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity.Journal of clinical biochemistry and nutrition · 2020Article
- Dietary nitrate attenuates high-fat diet-induced obesity via mechanisms involving higher adipocyte respiration and alterations in inflammatory status.Redox biology · 2020Article
- Brain Trauma Disrupts Hepatic Lipid Metabolism: Blame It on Fructose?Molecular nutrition & food research · 2019Article
- Inorganic nitrate, hypoxia, and the regulation of cardiac mitochondrial respiration-probing the role of PPARα.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in RatsFrontiers in pharmacology · 2019Article
- Nitrate and Nitrite in Health and Disease.Aging and disease · 2018Review
- Changes in arterial pressure and markers of nitric oxide homeostasis and oxidative stress following surgical correction of hydronephrosis in children.Pediatric nephrology (Berlin, Germany) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInorganic nitrate (NO3(-)) is a precursor of nitric oxide (NO) in the body and a large number of short-term studies with dietary nitrate supplementation in animals and humans show beneficial effects on cardiovascular health, exercise efficiency, host defense and ischemia reperfusion injury. In contrast, there is a long withstanding concern regarding the putative adverse effects of chronic nitrate exposure related to cancer and adverse hormonal effects. To address these concerns we performed in mice, a physiological and biochemical multi-analysis on the effects of long-term dietary nitrate supplementation.
design7 week-old C57BL/6 mice were put on a low-nitrate chow and at 20 weeks-old were treated with NaNO3 (1 mmol/L) or NaCl (1 mmol/L, control) in the drinking water. The groups were monitored for weight gain, food and water consumption, blood pressure, glucose metabolism, body composition and oxygen consumption until one group was reduced to eight animals due to death or illness. At that point remaining animals were sacrificed and blood and tissues were analyzed with respect to metabolism, cardiovascular function, inflammation, and oxidative stress.
resultsAnimals were supplemented for 17 months before final sacrifice. Body composition, oxygen consumption, blood pressure, glucose tolerance were measured during the experiment, and vascular reactivity and muscle mitochondrial efficiency measured at the end of the experiment with no differences identified between groups. Nitrate supplementation was associated with improved insulin response, decreased plasma IL-10 and a trend towards improved survival.
conclusionsLong term dietary nitrate in mice, at levels similar to the upper intake range in the western society, is not detrimental.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.