ArticleJournal of visualized experiments : JoVE2015
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 forFrontiers in immunology · 2024Article
- Simultaneous reduction of all ORMDL proteins decreases the threshold of mast cell activation.Scientific reports · 2023Article
- Neutrophil breaching of the blood vessel pericyte layer during diapedesis requires mast cell-derived IL-17A.Nature communications · 2022Article
- Mast Cells in the Mammalian Testis and Epididymis-Animal Models and Detection Methods.International journal of molecular sciences · 2022Review
- Multifaceted MRGPRX2: New insight into the role of mast cells in health and disease.The Journal of allergy and clinical immunology · 2021Review
- A Critical Role for NaJournal of immunology (Baltimore, Md. : 1950) · 2021Article
- Spontaneous atopic dermatitis in mice with a defective skin barrier is independent of ILC2 and mediated by IL-1β.Allergy · 2019Article
- The regulatory B cell-mediated peripheral tolerance maintained by mast cell IL-5 suppresses oxazolone-induced contact hypersensitivity.Science advances · 2019Article
- Brief Exposure of Skin to Near-Infrared Laser Modulates Mast Cell Function and Augments the Immune Response.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Degranulation of gastrointestinal mast cells contributes to hepatic ischemia-reperfusion injury in mice.Clinical science (London, England : 1979) · 2018Article
- Mast Cells and Innate Lymphoid Cells: Underappreciated Players in CNS Autoimmune Demyelinating Disease.Frontiers in immunology · 2018Review
- Imaging protective mast cells in living mice during severe contact hypersensitivity.JCI insight · 2017Article
- Article
- Pathways of immediate hypothermia and leukocyte infiltration in an adjuvant-free mouse model of anaphylaxis.The Journal of allergy and clinical immunology · 2017Article
- A TNFRSF14-FcɛRI-mast cell pathway contributes to development of multiple features of asthma pathology in mice.Nature communications · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Mast cells (MCs) are hematopoietic cells which reside in various tissues, and are especially abundant at sites exposed to the external environment, such as skin, airways and gastrointestinal tract. Best known for their detrimental role in IgE-dependent allergic reactions, MCs have also emerged as important players in host defense against venom and invading bacteria and parasites. MC phenotype and function can be influenced by microenvironmental factors that may differ according to anatomic location and/or based on the type or stage of development of immune responses. For this reason, we and others have favored in vivo approaches over in vitro methods to gain insight into MC functions. Here, we describe methods for the generation of mouse bone marrow-derived cultured MCs (BMCMCs), their adoptive transfer into genetically MC-deficient mice, and the analysis of the numbers and distribution of adoptively transferred MCs at different anatomical sites. This method, named the 'mast cell knock-in' approach, has been extensively used over the past 30 years to assess the functions of MCs and MC-derived products in vivo. We discuss the advantages and limitations of this method, in light of alternative approaches that have been developed in recent years.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.