ArticleScientific reports2015
Biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in Escherichia coli.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 113 citations in OpenAlex.
- A Putative Multicopper Oxidase Encoded byInternational journal of molecular sciences · 2026Article
- Characterization and application potential of a robust recombinant laccase rTpLcc3 from Trametes polyzona for textile dye decolorization.Scientific reports · 2026Article
- Cloning and Heterologous Expression of Laccase from Beauveria pseudobassiana PHF4 in E. coli and its Application in the Degradation of Catechol.Current microbiology · 2026Article
- Machine Learning Prediction of Laccase-Catalyzed Oxidation of Aromatic Compounds Using Curated Enzyme-Specific Datasets.Journal of computational chemistry · 2026Article
- Catalytically active inclusion bodies of Bacillus clausii laccase protein recombinantly produced in E. coli for dye decolorization.Microbial cell factories · 2026Article
- Lignocellulose degradation in bacteria and fungi: cellulosomes and industrial relevance.Frontiers in microbiology · 2025Review
- Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds.Biotechnology letters · 2024Article
- Degradation of tetracycline, oxytetracycline & ampicillin by purified multiple copper oxidase like laccase from Stentrophomonas sp. YBX1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Effects of Orange Peel Extract on Laccase Activity and Gene Expression inJournal of fungi (Basel, Switzerland) · 2024Article
- Bacterial transformation of lignin: key enzymes and high-value products.Biotechnology for biofuels and bioproducts · 2024Review
- An Inferred Ancestral CotA Laccase with Improved Expression and Kinetic Efficiency.International journal of molecular sciences · 2023Article
- Microaerobic conditions enhance laccase production from Rheinheimera sp. in an economical medium.Archives of microbiology · 2022Article
- Current Advances in Biodegradation of Polyolefins.Microorganisms · 2022Review
- Genomics analysis and degradation characteristics of lignin by Streptomyces thermocarboxydus strain DF3-3.Biotechnology for biofuels and bioproducts · 2022Article
- Current Progress and Future Perspectives on the Use of Bacillus clausiiMicroorganisms · 2022Review
- Conversion of lignin-derived 3-methoxycatechol to the natural product purpurogallin using bacterial P450 GcoAB and laccase CueO.Applied microbiology and biotechnology · 2022Article
- Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase fromFood technology and biotechnology · 2021Article
- A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases.Molecules (Basel, Switzerland) · 2021Review
- Genome-based engineering of ligninolytic enzymes in fungi.Microbial cell factories · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense of molecular oxygen, without any need for co-factor regeneration. These enzymes bear high potential for the sustainable synthesis of fine chemicals and the modification of (bio)polymers. Here we describe cloning and expression of five novel bacterial laccase-like multi copper oxidases (LMCOs) of diverse origin which were identified by homology searches in online databases. Activity yields under different expression conditions and temperature stabilities were compared to three previously described enzymes from Bacillus subtilis, Bacillus pumilus and Bacillus clausii. In almost all cases, a switch to oxygen-limited growth conditions after induction increased volumetric activity considerably. For proteins with predicted signal peptides for secretion, recombinant expression with and without signal sequence was investigated. Bacillus CotA-type LMCOs outperformed enzymes from Streptomyces and Gram-negative bacteria with respect to activity yields in Escherichia coli and application relevant biochemical properties. The novel Bacillus coagulans LMCO combined high activity yields in E. coli with unprecedented activity at strong alkaline pH and high storage stability, making it a promising candidate for further development.
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