ArticleMicrobiology (Reading, England)2015
Mycobacteriophage-repressor-mediated immunity as a selectable genetic marker: Adephagia and BPs repressor selection.
Article in Microbiology (Reading, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- All the world's a phage.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Prophage ϕSA169 Enhances Vancomycin Persistence in Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Mycobacteriophage Yasnaya_Polyana and its engineered lytic derivative: specificity of regulatory motifs and lytic potential.Frontiers in microbiology · 2025Article
- Beyond Antibiotics: Exploring the Potential of Bacteriophages and Phage Therapy.PHAGE (New Rochelle, N.Y.) · 2024Review
- Characterization of mycobacteriophage Adephagia cytotoxic proteins.G3 (Bethesda, Md.) · 2024Article
- Article
- Characterization of Temperate LPS-Binding Bordetella avium Phages That Lack Superinfection Immunity.Microbiology spectrum · 2023Article
- Mycobacteriophages: From Petri dish to patient.PLoS pathogens · 2022Review
- Phylogenetic relationships and codon usage bias amongst cluster K mycobacteriophages.G3 (Bethesda, Md.) · 2021Article
- Wildy Prize Lecture, 2020-2021: Who wouldn't want to discover a new virus?Microbiology (Reading, England) · 2021Article
- The Prophage and Plasmid Mobilome as a Likely Driver of Mycobacterium abscessus Diversity.mBio · 2021Article
- Mycobacteriophages as Potential Therapeutic Agents against Drug-Resistant Tuberculosis.International journal of molecular sciences · 2021Review
- Actinobacteriophages: Genomics, Dynamics, and Applications.Annual review of virology · 2020Review
- Whole genome sequencing identifies an allele responsible for clear vs. turbid plaque morphology in a Mycobacteriophage.BMC microbiology · 2020Article
- Evolutionary entanglement of mobile genetic elements and host defence systems: guns for hire.Nature reviews. Genetics · 2020Review
- Article
- Mycobacteriophage ZoeJ: A broad host-range close relative of mycobacteriophage TM4.Tuberculosis (Edinburgh, Scotland) · 2019Article
- Mycobacteriophages.Microbiology spectrum · 2018Review
- Fluorescent Reporter DS6A Mycobacteriophages Reveal Unique Variations in Infectibility and Phage Production in Mycobacteria.Journal of bacteriology · 2016Article
- Complete Genome Sequence of the Streptomyces Phage Nanodon.Genome announcements · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mycobacteriophages provide an abundance of systems for use in mycobacterial genetics, including manipulation of Mycobacterium tuberculosis. Because of the dearth of antibiotic resistance cassettes and biosafety concerns in constructing recombinant virulent M. tuberculosis strains, we developed the use of mycobacteriophage-encoded repressor genes that can be selected in the presence of lytic versions of their cognate phages. The phage Adephagia repressor gene (43) was identified through its ability to confer immunity to Adephagia superinfection, together with the mapping of mutations in gene 43 that confer a clear-phage phenotype. Plasmid transformants containing either Adephagia 43 or the previously identified BPs repressor 33 can be readily selected following electroporation using engineered lytic derivatives of Adephagia and BPs, respectively. Selection is as efficient as antibiotic selection, can be used with either single-copy integration vectors or with extrachromosomal vectors, and works similarly in both Mycobacterium smegmatis and M. tuberculosis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.