ArticleJournal of visualized experiments : JoVE2015
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Pathobionts from chemically disrupted gut microbiota induce insulin-dependent diabetes in mice.Microbiome · 2023Article
- Intrahepatic microbes govern liver immunity by programming NKT cells.The Journal of clinical investigation · 2022Article
- A murine periodontitis model using coaggregation between human pathogens and a predominant mouse oral commensal bacterium.Journal of periodontal & implant science · 2022Article
- Translocation of vaginal microbiota is involved in impairment and protection of uterine health.Nature communications · 2021Observational
- Visualization of Respiratory Commensal Bacteria in Context of Their Natural Host Environment.Frontiers in microbiology · 2021Article
- Article
- Innate αβ T Cells Mediate Antitumor Immunity by Orchestrating Immunogenic Macrophage Programming.Cancer discovery · 2019Article
- The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression.Cancer discovery · 2018Article
- Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis.Journal of visualized experiments : JoVE · 2018Article
- Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions.Journal of dental research · 2018Article
- The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.Scientific reports · 2016Article
- An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue.BMC research notes · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of bacteria within the pocket epithelium and underlying connective tissue in gingival biopsies from patients with periodontitis has been reported using various methods, including electron microscopy, immunohistochemistry or immunofluorescence using bacteria-specific antibodies, and fluorescent in situ hybridization (FISH) using a fluorescence-labeled oligonucleotide probe. Nevertheless, these methods are not widely used due to technical limitation or difficulties. Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled DNA probes has been introduced. The paraffin-embedded tissues are the most common form of biopsy tissues available from pathology banks. Bacteria can be detected either in a species-specific or universal manner. Bacterial signals are detected as either discrete forms (coccus, rod, fusiform, and hairy form) of bacteria or dispersed forms. The technique allows other histological information to be obtained: the epithelia, connective tissue, inflammatory infiltrates, and blood vessels are well distinguished. This method can be used to study the role of bacteria in various diseases, such as periodontitis, cancers, and inflammatory immune diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.