ArticleJournal of visualized experiments : JoVE2015
Do's and don'ts in the preparation of muscle cryosections for histological analysis.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- VBIT-4 Rescues Mitochondrial Dysfunction and Reduces Skeletal Muscle Degeneration in a Severe Model of Duchenne Muscular Dystrophy.International journal of molecular sciences · 2025Article
- Neuromuscular junction visualization in paraffin-embedded thyroarytenoid muscle sections: Expanding options beyond frozen section analysis.Laryngoscope investigative otolaryngology · 2025Article
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- Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds.Animals : an open access journal from MDPI · 2024Article
- Reduction of Mitochondrial Calcium Overload via MKT077-Induced Inhibition of Glucose-Regulated Protein 75 Alleviates Skeletal Muscle Pathology in Dystrophin-DeficientInternational journal of molecular sciences · 2024Article
- Multimodal nanoimmunotherapy engages neutrophils to eliminate Staphylococcus aureus infections.Nature nanotechnology · 2024Article
- Development of a Low-cost Epimysial Electromyography Electrode: A Simplified Workflow for Fabrication and Testing.Journal of visualized experiments : JoVE · 2024Article
- Mitochondrial Transplantation Therapy Ameliorates Muscular Dystrophy inBiomolecules · 2024Article
- An optimized approach to study nanoscale sarcomere structure utilizing super-resolution microscopy with nanobodies.PloS one · 2024Article
- Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions.Physiological reviews · 2023Review
- Histological Methods to Assess Skeletal Muscle Degeneration and Regeneration in Duchenne Muscular Dystrophy.International journal of molecular sciences · 2022Review
- Tissue-specific Grb10/Ddc insulator drives allelic architecture for cardiac development.Molecular cell · 2022Article
- State of Knowledge on Molecular Adaptations to Exercise in Humans: Historical Perspectives and Future Directions.Comprehensive Physiology · 2022Article
- Effect of resistance training on satellite cells in old mice - a transcriptome study : implications for sarcopenia.Bone & joint research · 2022Article
- UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle.Frontiers in physiology · 2022Article
- Effects of Vocal Training on Thyroarytenoid Muscle Neuromuscular Junctions and Myofibers in Young and Older Rats.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- Preparation of the Rat Vocal Fold for Neuromuscular Analyses.Journal of visualized experiments : JoVE · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histological evaluation of muscle biopsies has served as an indispensable tool in the understanding of the development and progression of pathology of neuromuscular disorders. However, in order to do so, proper care needs to be taken when excising and preserving tissues to achieve optimal staining. One method of tissue preservation involves fixing tissues in formaldehyde and then embedding them with paraffin wax. This method preserves morphology well and allows for long-term storage at RT but is cumbersome and requires handling of toxic chemicals. Further, formaldehyde fixation results in antigen cross-linking, which necessitates antigen retrieval protocols for effective immunostaining. On the contrary, frozen sectioning does not require fixation and thus retains biological antigen conformation. This method also provides a distinct advantage in quick turn around time, making it especially useful in situations needing quick histological evaluation like intraoperative surgical biopsies. Here we describe the most effective method of preparing muscle biopsies for visualization with different histological and immunological stains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.