Evidence map›Paper›PMID 26065859›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015

The fatty acid desaturase 2 (FADS2) gene product catalyzes Δ4 desaturation to yield n-3 docosahexaenoic acid and n-6 docosapentaenoic acid in human cells.

Hui Gyu Park, Woo Jung Park, Kumar S D Kothapalli, J Thomas Brenna

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed, 126 citations in OpenAlex.

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  14. AreACS omega · 2022
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15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Hui Gyu Park*Division of Nutritional Sciences and Department of Food Science, Cornell University, Ithaca, New York, USA; and Department of Marine Food Science and Technology, Gangneung-Wonju National University, South Korea.
Woo Jung Park*Division of Nutritional Sciences and Department of Food Science, Cornell University, Ithaca, New York, USA; and Department of Marine Food Science and Technology, Gangneung-Wonju National University, South Korea.
Kumar S D Kothapalli*Division of Nutritional Sciences and Department of Food Science, Cornell University, Ithaca, New York, USA; and Department of Marine Food Science and Technology, Gangneung-Wonju National University, South Korea jtb4@cornell.edu ksk25@cornell.edu.
J Thomas Brenna*Division of Nutritional Sciences and Department of Food Science, Cornell University, Ithaca, New York, USA; and Department of Marine Food Science and Technology, Gangneung-Wonju National University, South Korea jtb4@cornell.edu ksk25@cornell.edu.
Cornell University · US

Funding

Molecular mechanism of omega-3 responseR01AT007003 · NCCIH · UNIVERSITY OF TEXAS AT AUSTIN · PI BRENNA, JAMES T. · 2013 to 2017
$1.9M
NCCIH NIH HHS R01 AT007003
6 · The paper itself

Abstract

Docosahexaenoic acid (DHA) is a Δ4-desaturated C22 fatty acid and the limiting highly unsaturated fatty acid (HUFA) in neural tissue. The biosynthesis of Δ4-desaturated docosanoid fatty acids 22:6n-3 and 22:5n-6 are believed to proceed via a circuitous biochemical pathway requiring repeated use of a fatty acid desaturase 2 (FADS2) protein to perform Δ6 desaturation on C24 fatty acids in the endoplasmic reticulum followed by 1 round of β-oxidation in the peroxisomes. We demonstrate here that the FADS2 gene product can directly Δ4-desaturate 22:5n-3→22:6n-3 (DHA) and 22:4n-6→22:5n-6. Human MCF-7 cells lacking functional FADS2-mediated Δ6-desaturase were stably transformed with FADS2, FADS1, or empty vector. When incubated with 22:5n-3 or 22:4n-6, FADS2 stable cells produce 22:6n-3 or 22:5n-6, respectively. Similarly, FADS2 stable cells when incubated with d5-18:3n-3 show synthesis of d5-22:6n-3 with no labeling of 24:5n-3 or 24:6n-3 at 24 h. Further, both C24 fatty acids are shown to be products of the respective C22 fatty acids via elongation. Our results demonstrate that the FADS2 classical transcript mediates direct Δ4 desaturation to yield 22:6n-3 and 22:5n-6 in human cells, as has been widely shown previously for desaturation by fish and many other organisms.

Indexed as

AnimalsCatalysisCell Line, TumorDelta-5 Fatty Acid DesaturaseDocosahexaenoic AcidsFatty Acid DesaturasesFatty Acids, UnsaturatedHumansPapioDelta-5 Fatty Acid DesaturaseDocosahexaenoic Acidsdocosapentaenoic acidFADS1 protein, humanFADS2 protein, humanFatty Acid DesaturasesFatty Acids, Unsaturatedmetabolismnutritionpolyunsaturated fatty acidsΔ6 desaturation

Identifiers

PMID26065859
PMCPMC4550368
OpenAlexW1818048492

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.