ArticleScientific reports2015
The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
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- Factors Influencing Venous Remodeling in the Development of Varicose Veins of the Lower Limbs.International journal of molecular sciences · 2024Review
- The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein-A Preliminary Study.Biomedicines · 2023Article
- Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces.World journal of stem cells · 2022Article
- Modulation of Cholesterol Pathways in Human Macrophages Infected by Clinical Isolates ofFrontiers in cellular and infection microbiology · 2022Article
- Therapeutic potential of human induced pluripotent stem cells and renal progenitor cells in experimental chronic kidney disease.Stem cell research & therapy · 2020Article
- A mechanical non-enzymatic method for isolation of mouse embryonic fibroblasts.Molecular biology reports · 2020Article
- Nanofibers as Bioinstructive Scaffolds Capable of Modulating Differentiation through Mechanosensitive Pathways for Regenerative Engineering.Regenerative engineering and translational medicine · 2019Article
- Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics.Stem cell reports · 2018Article
- A Contact-Based Method for Differentiation of Human Mesenchymal Stem Cells into an Endothelial Cell-Phenotype.Cell biochemistry and biophysics · 2018Article
- Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitro.Scientific reports · 2018Article
- Synthesis and characterization of a photocleavable collagen-like peptide.Organic & biomolecular chemistry · 2018Article
- The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons.Stem cells international · 2018Article
- Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters.Biology open · 2016Article
- Development of functionalized multi-walled carbon-nanotube-based alginate hydrogels for enabling biomimetic technologies.Scientific reports · 2016Article
- Supramolecular Nanofibers Enhance Growth Factor Signaling by Increasing Lipid Raft Mobility.Nano letters · 2016Article
- Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro.Development & reproduction · 2015Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological activity of cell-derived substrates to maintain undifferentiated murine-induced pluripotent stem (iPS) cells was correlated to membrane fluidity as a new parameter of cell culture substrates. Murine embryonic fibroblasts (MEFs) were employed as feeder cells and their membrane fluidity was tuned by chemical fixation using formaldehyde (FA). Membrane fluidity was evaluated by real-time single-molecule observations of green fluorescent protein-labeled epidermal growth factor receptors on chemically fixed MEFs. Biological activity was monitored by colony formation of iPS cells. Treatment with a low concentration of FA sustained the membrane fluidity and biological activity, which were comparable to those of mitomycin C-treated MEFs. The biological activity was further confirmed by sustained expression of alkaline phosphatase, SSEA-1, and other pluripotency markers in iPS cells after 3-5 days of culture on FA-fixed MEFs. Chemical fixation of feeder cells has several advantages such as providing ready-to-use culture substrates without contamination by proliferating feeder cells. Therefore, our results provide an important basis for the development of chemically fixed culture substrates for pluripotent stem cell culture as an alternative to conventional treatment by mitomycin C or x-ray irradiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.