ArticleScientific reports2015
High resolution imaging of intracellular oxygen concentration by phosphorescence lifetime.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 127 citations in OpenAlex.
- Advanced multimodal imaging: FLIM, PLIM, and FluoRaman enabled by novel diarylacetylene probes.The Analyst · 2026Article
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- The Biophysics of Flash Radiotherapy: Tools for Measuring Tumor and Normal Tissues Microenvironment.Antioxidants (Basel, Switzerland) · 2025Review
- Combining Photodynamic Therapy and Targeted Drug Delivery Systems: Enhancing Mitochondrial Toxicity for Improved Cancer Outcomes.International journal of molecular sciences · 2024Review
- Real-Time Monitoring of Oxygen-Consumption Rate in Mouse Liver Slices Incubated in Organ-on-a-Chip Devices.Analytical chemistry · 2024Article
- Cyanine dyes in the mitochondria-targeting photodynamic and photothermal therapy.Communications chemistry · 2024Review
- Tutorial on compressed ultrafast photography.Journal of biomedical optics · 2024Review
- The Dual Luminescence Lifetime pH/Oxygen Sensor: Evaluation of Applicability for Intravital Analysis of 2D- and 3D-Cultivated Human Endometrial Mesenchymal Stromal Cells.International journal of molecular sciences · 2023Article
- Three-dimensional oxygen concentration monitoring in hydrogels using low-cost phosphorescence lifetime imaging for tissue engineering.Biomedical optics express · 2023Article
- Proton FLASH effects on mouse skin at different oxygen tensions.Physics in medicine and biology · 2023Article
- Oxaloacetate enhances and accelerates regeneration in young mice by promoting proliferation and mineralization.Frontiers in cell and developmental biology · 2023Article
- Computational Modeling and Imaging of the Intracellular Oxygen Gradient.International journal of molecular sciences · 2022Article
- A first principles examination of phosphorescence.RSC advances · 2022Article
- Spatial transcriptomics reveals metabolic changes underly age-dependent declines in digit regeneration.eLife · 2022Article
- Review
- Review
- Fast wide-field upconversion luminescence lifetime thermometry enabled by single-shot compressed ultrahigh-speed imaging.Nature communications · 2021Article
- An effectiveNanoscale advances · 2021Article
- Ru(ii)/BODIPY core co-encapsulated ratiometric nanotools for intracellular ORSC chemical biology · 2021Article
- Regulation of Oxygen Homeostasis at the Intestinal Epithelial Barrier Site.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical methods using phosphorescence quenching by oxygen are suitable for sequential monitoring and non-invasive measurements for oxygen concentration (OC) imaging within cells. Phosphorescence intensity measurement is widely used with phosphorescent dyes. These dyes are ubiquitously but heterogeneously distributed inside the whole cell. The distribution of phosphorescent dye is a major disadvantage in phosphorescence intensity measurement. We established OC imaging system for a single cell using phosphorescence lifetime and a laser scanning confocal microscope. This system had improved spatial resolution and reduced the measurement time with the high repetition rate of the laser. By the combination of ubiquitously distributed phosphorescent dye with this lifetime imaging microscope, we can visualize the OC inside the whole cell and spheroid. This system uses reversible phosphorescence quenching by oxygen, so it can measure successive OC changes from normoxia to anoxia. Lower regions of OC inside the cell colocalized with mitochondria. The time-dependent OC change in an insulin-producing cell line MIN6 by the glucose stimulation was successfully visualized. Assessing the detailed distribution and dynamics of OC inside cells achieved by the presented system will be useful to understanding a physiological and pathological oxygen metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.