Evidence map›Paper›PMID 26064010›Full record

ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2015

Detection of Theileria annulata in blood samples of native cattle by PCR and smear method in Southeast of Iran.

Saeid R Nourollahi-Fard, Mohammad Khalili, Nima Ghalekhani

Open access · bronzeAbstract read
In one paragraph

Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Molecular detection and characterization ofParasite epidemiology and control · 2024
    Article
  5. Article
  6. Article
  7. Genetic Resistance of Bovines to Theileriosis.Animals : an open access journal from MDPI · 2022
    Review
  8. Article
  9. Article
  10. Molecular Epidemiology ofAnimals : an open access journal from MDPI · 2021
    Article
  11. Article
  12. Article
  13. Article
  14. Detection of theileriosis in cattle and buffaloes by polymerase chain reaction.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Saeid R Nourollahi-FardDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Mohammad KhaliliDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Nima GhalekhaniDVM, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Shahid Bahonar University of Kerman · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Theileria annulata, a protozoan parasite of cattle is causes tropical theileriosis. Polymerase chain reaction (PCR) was used to assess the presence and the frequency of T. annulata infection in blood samples obtained from carrier cattle in Kerman, Southeast of Iran. Blood samples were collected in citrate solution from 150 native cattle with mean age of 1 year which selected randomly. Primarily, a thin layer smear was prepared from their ear sublime vein blood and was fixed with methanol and stained with Giemsa dye. Blood smears were examined for the presence of parasites, and blood samples were analyzed by PCR. Piroplasmic forms of T. annulata were seen in 16 of 150 (10.66 %) by examination the blood smears with light microscope, whereas 68 of 150 (45.33 %) cattle were positive by PCR method. All animals that were positive by blood smears were also positive by PCR. Difference between these methods was significant (P < 0.05). Our results demonstrate that this PCR assay in diagnosing T. annulata parasites in carrier cattle is more sensitive than method of smear preparation and can be used in epidemiological studies.

Indexed as

CattlePCRSmear methodTheileria annulata

Identifiers

PMID26064010
PMCPMC4456547
OpenAlexW2010873305

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.