Evidence map›Paper›PMID 26063995›Full record

ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2015

Comparative efficacy of different in vitro cultivation media for Trypanosoma evansi isolated from different mammalian hosts inhabiting different geographical areas of India.

Rajender Kumar, Jarnail Singh, Rubi Singh, Sanjay Kumar, S C Yadav

Abstract read
In one paragraph

Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023
    Article
  4. Article
  5. Article
  6. Molecular Characterization ofFrontiers in cellular and infection microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rajender KumarNational Research Centre on Equines, Sirsa Road, Hisar, 125001 Haryana India.
Jarnail SinghNational Research Centre on Equines, Sirsa Road, Hisar, 125001 Haryana India.
Rubi SinghNational Research Centre on Equines, Sirsa Road, Hisar, 125001 Haryana India.
Sanjay KumarNational Research Centre on Equines, Sirsa Road, Hisar, 125001 Haryana India.
S C YadavNational Research Centre on Equines, Sirsa Road, Hisar, 125001 Haryana India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study was undertaken to establish an optimal medium for primary culture initiation and maintenance of T. evansi isolated from different mammalian hosts of diverse geographical regions of India viz. donkey/1 (Hardoi, Uttar Pradesh), donkey/2 (Junagarh, Gujarat), pony/1 (Hisar, Haryana), camel/1 (Bikaner, Rajasthan) which represented isolates 1, 2, 3 and 4, respectively. Primary cultures were initiated with all four isolates in five different in vitro cultivation media with seeding density of 1 × 10(6) trypanosomes/ml. The parasites of all four isolates could remain viable only for 48 h in medium E (Alsever's solution) and for 72 h in medium A, C and D. Parasites reached to a maximum density (2.5-3.75 × 10(6)/ml) within 24 h and thereafter, a sharp decline (0.5-0.75 × 10(6)/ml) in the next 72 h was observed in 1, 2 and 3 isolates cultured in medium B. In isolate 4, parasite counts got more than doubled in 24 h and then decreased gradually up to sixth day post initiation of cultivation which thereafter increased gradually up to 34 days and a constant parasite number of 10(5)/ml could be achieved for 90 days in medium B. During this prolonged culture the trypanosomes retained their long slender morphology and infectivity to mice.

Indexed as

Culture mediaIn vitro cultivationLivestockTrypanosoma evansi isolates

Identifiers

PMID26063995
PMCPMC4456538

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.