ArticleIndian journal of microbiology2015
Genome Wide Analysis for Searching Novel Markers to Rapidly Identify Clostridium Strains.
Article in Indian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Aligning Microbial Biodiversity for Valorization of Biowastes: Conception to Perception.Indian journal of microbiology · 2019Review
- Rapid Detection Device forIndian journal of microbiology · 2018Article
- Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease.Indian journal of microbiology · 2017Article
- Comparative Genomics Reveals Biomarkers to Identify Lactobacillus Species.Indian journal of microbiology · 2016Article
- An In Silico Approach for Identification of the Pathogenic Species, Helicobacter pylori and Its Relatives.Indian journal of microbiology · 2016Article
- Identification of DNA Methyltransferase Genes in Human Pathogenic Bacteria by Comparative Genomics.Indian journal of microbiology · 2016Article
- Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification.Indian journal of microbiology · 2016Article
- A Genome-Wide Profiling Strategy as an Aid for Searching Unique Identification Biomarkers for Streptococcus.Indian journal of microbiology · 2016Article
- Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria.Indian journal of microbiology · 2016Review
- Genome Wide Search for Biomarkers to Diagnose Yersinia Infections.Indian journal of microbiology · 2015Article
- Genome Wide Analysis for Rapid Identification of Vibrio Species.Indian journal of microbiology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial classification is based largely on the 16S rRNA (rrs) gene sequence, which is conserved throughout the prokaryotic domain. The Ribosomal Database Project (RDP) has become a reference point for almost all practical purposes. The use of this gene is limited by the fact that it can be used to identify only to the extent to what has been known and is available in the RDP. In order to identify an organism whose rrs is not present in the RDP database, we need to generate novel markers to place the unknown on the evolutionary map. Here, sequenced genomes of 27 Clostridium strains belonging to 9 species have been used to identify two sets of genes: (1) common to most of the species, and (2) unique to a species. Combinations of genes (recN, dnaJ, secA, mutS, and/or grpE) and their unique restriction endonuclease digestion (AluI, BfaI and/or Tru9I) patterns have been established to rapidly identify Clostridium species. This strategy for identifying novel markers can be extended to all other organisms and diagnostic applications.
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