ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Neural activity selects myosin IIB and VI with a specific time window in distinct dynamin isoform-mediated synaptic vesicle reuse pathways.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- ERICH3: vesicular association and antidepressant treatment response.Molecular psychiatry · 2021Pooled it
- Proteomic profiling across early postnatal development of midbrain dopamine axons innervating the dorsal striatum.bioRxiv : the preprint server for biology · 2026Article
- Optimal neuromuscular performance requires motor neuron phosphagen kinases.The Journal of physiology · 2026Article
- The lemur tail kinase family in neuronal function and disfunction in neurodegenerative diseases.Cellular and molecular life sciences : CMLS · 2024Review
- Article
- Mechanisms of Synaptic Vesicle Exo- and Endocytosis.Biomedicines · 2022Review
- Neurotransmitter Release Site Replenishment and Presynaptic Plasticity.International journal of molecular sciences · 2020Review
- Synthesis and Evaluation of 4-Hydroxycoumarin Imines as Inhibitors of Class II Myosins.Journal of medicinal chemistry · 2020Article
- A Tripartite Interaction Among the Calcium Channel αFrontiers in cellular neuroscience · 2019Article
- Article
- The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System.Frontiers in molecular neuroscience · 2017Article
- Millisecond CaProceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- Myosin accelerates synaptic vesicle recycling in the motor nerve endings.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2016Article
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presynaptic nerve terminals must maintain stable neurotransmissions via synaptic vesicle (SV) resupply despite encountering wide fluctuations in the number and frequency of incoming action potentials (APs). However, the molecular mechanism linking variation in neural activity to SV resupply is unknown. Myosins II and VI are actin-based cytoskeletal motors that drive dendritic actin dynamics and membrane transport, respectively, at brain synapses. Here we combined genetic knockdown or molecular dysfunction and direct physiological measurement of fast synaptic transmission from paired rat superior cervical ganglion neurons in culture to show that myosins IIB and VI work individually in SV reuse pathways, having distinct dependency and time constants with physiological AP frequency. Myosin VI resupplied the readily releasable pool (RRP) with slow kinetics independently of firing rates but acted quickly within 50 ms after AP. Under high-frequency AP firing, myosin IIB resupplied the RRP with fast kinetics in a slower time window of 200 ms. Knockdown of both myosin and dynamin isoforms by mixed siRNA microinjection revealed that myosin IIB-mediated SV resupply follows amphiphysin/dynamin-1-mediated endocytosis, while myosin VI-mediated SV resupply follows dynamin-3-mediated endocytosis. Collectively, our findings show how distinct myosin isoforms work as vesicle motors in appropriate SV reuse pathways associated with specific firing patterns.
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