ArticleJournal of clinical microbiology2015
Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Clinical Characteristics of Enterovirus D68 Respiratory Disease in Colorado Children Over a Decade: 2014-2025.The Pediatric infectious disease journal · 2026Article
- Implementation and outcomes of a rapid response genomic hospital epidemiology programme at an academic medical centre over 7 years.The Lancet. Microbe · 2026Review
- Respiratory enterovirus D68: virology, clinical surveillance, host-pathogen interactions, and therapeutic prospects.Frontiers in cellular and infection microbiology · 2026Review
- A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance.Diagnostic microbiology and infectious disease · 2025Article
- Type-specific EV-D68 real-time RT-PCR assay for the detection of all extant enterovirus D68 strains.Journal of clinical microbiology · 2025Article
- Article
- Optimizing RT-qPCR multiplex assays for simultaneous detection of enteric and respiratory viruses in wastewater.Journal of water and health · 2025Article
- Multimodal Surveillance Model for Enterovirus D68 Respiratory Disease and Acute Flaccid Myelitis among Children in Colorado, USA, 2022.Emerging infectious diseases · 2024Review
- Trends of Enterovirus D68 Concentrations in Wastewater, California, USA, February 2021-April 2023.Emerging infectious diseases · 2023Article
- Qualitative detection of enterovirus D68 from PrimeStore® molecular transport medium: implications for home- and self-collection.Diagnostic microbiology and infectious disease · 2023Article
- Telaprevir Treatment Reduces Paralysis in a Mouse Model of Enterovirus D68 Acute Flaccid Myelitis.Journal of virology · 2023Article
- Article
- Development of Novel PCR Assays for Improved Detection of Enterovirus D68.Journal of clinical microbiology · 2021Article
- A Case Report of Acute Flaccid Paralysis Caused by Enterovirus D68 Infection: The Beginning of a Polio-Like Epidemic?Cureus · 2021Article
- Biennial Upsurge and Molecular Epidemiology of Enterovirus D68 Infection in New York, USA, 2014 to 2018.Journal of clinical microbiology · 2020Article
- Acute Flaccid Myelitis in Children in Zhejiang Province, China.Frontiers in neurology · 2020Article
- Enterovirus D68 respiratory infection in a children's hospital in Japan in 2015.Pediatrics international : official journal of the Japan Pediatric Society · 2019Article
- Development of a respiratory disease model for enterovirus D68 in 4-week-old mice for evaluation of antiviral therapies.Antiviral research · 2019Article
- Article
- Enterovirus D68 Surveillance, St. Louis, Missouri, USA, 2016.Emerging infectious diseases · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have developed and evaluated a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (EV-D68) in clinical specimens. This assay was developed in response to the unprecedented 2014 nationwide EV-D68 outbreak in the United States associated with severe respiratory illness. As part of our evaluation of the outbreak, we sequenced and published the genome sequence of the EV-D68 virus circulating in St. Louis, MO. This sequence, along with other GenBank sequences from past EV-D68 occurrences, was used to computationally select a region of EV-D68 appropriate for targeting in a strain-specific RT-PCR assay. The RT-PCR assay amplifies a segment of the VP1 gene, with an analytic limit of detection of 4 copies per reaction, and it was more sensitive than commercially available assays that detect enteroviruses and rhinoviruses without distinguishing between the two, including three multiplex respiratory panels approved for clinical use by the FDA. The assay did not detect any other enteroviruses or rhinoviruses tested and did detect divergent strains of EV-D68, including the first EV-D68 strain (Fermon) identified in California in 1962. This assay should be useful for identifying and studying current and future outbreaks of EV-D68 viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.