ArticleJournal of clinical microbiology2015
Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- TrackingACS omega · 2026Review
- Non-Targeted Screening Method for Detecting Temporal Shifts in Spectral Patterns of Methicillin-ResistantMicroorganisms · 2026Article
- Bacterial infections and pathogenic situations: The case ofVirulence · 2025Review
- A MALDI-ToF mass spectrometry database for identification and classification of highly pathogenic bacteria.Scientific data · 2025Article
- Lipidomics Characterization of the Microbiome in People with Diabetic Foot Infection Using MALDI-TOF MS.Analytical chemistry · 2023Article
- Discrimination ofMicroorganisms · 2021Article
- MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives.Chemistry, an Asian journal · 2021Review
- Protein biomarker elucidation for the verification of biological agents in the taxonomic group of Gammaproteobacteria using tandem mass spectrometry.World journal of microbiology & biotechnology · 2021Article
- Yersinia pestis: the Natural History of Plague.Clinical microbiology reviews · 2020Review
- Identification of Microorganisms by Liquid Chromatography-Mass Spectrometry (LC-MSMolecular & cellular proteomics : MCP · 2020Article
- MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks.Scientific reports · 2020Article
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification of security-sensitive bacteria: Considerations for Canadian Bruker users.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2020Article
- Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library.PLoS neglected tropical diseases · 2020Article
- Recent applications of mass spectrometry in bacterial lipidomics.Analytical and bioanalytical chemistry · 2020Review
- Proteomics ofMicroorganisms · 2020Article
- Elucidation of protein biomarkers for verification of selected biological warfare agents using tandem mass spectrometry.Scientific reports · 2020Article
- Profiling for Bioactive Peptides and Volatiles of Plant Growth Promoting Strains of theFrontiers in microbiology · 2020Article
- MALDI-MS: Emerging roles in pathology and laboratory medicine.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids.Nature microbiology · 2019Article
- Review of the impact of MALDI-TOF MS in public health and hospital hygiene, 2018.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the case of a release of highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, and genotyping of HPB. In the present study, the results of a joint exercise with 11 partner institutions from nine European countries are presented. In this exercise, 10 distinct microbial samples, among them five HPB, Bacillus anthracis, Brucella canis, Burkholderia mallei, Burkholderia pseudomallei, and Yersinia pestis, were characterized under blinded conditions. Microbial strains were inactivated by high-dose gamma irradiation before shipment. Preparatory investigations ensured that this type of inactivation induced only subtle spectral changes with negligible influence on the quality of the diagnosis. Furthermore, pilot tests on nonpathogenic strains were systematically conducted to ensure the suitability of sample preparation and to optimize and standardize the workflow for microbial identification. The analysis of the microbial mass spectra was carried out by the individual laboratories on the basis of spectral libraries available on site. All mass spectra were also tested against an in-house HPB library at the Robert Koch Institute (RKI). The averaged identification accuracy was 77% in the first case and improved to >93% when the spectral diagnoses were obtained on the basis of the RKI library. The compilation of complete and comprehensive databases with spectra from a broad strain collection is therefore considered of paramount importance for accurate microbial identification.
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