ArticleThe Journal of biological chemistry2015
The Levels of a Universally Conserved tRNA Modification Regulate Cell Growth.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Catalytic and regulatory basis of tRNA tNature communications · 2026Article
- Tissue-specific role of dTrmO in threonine decoding during Drosophila melanogaster development.Biological research · 2026Article
- Structures of KEOPS bound to tRNA reveal functional roles of the kinase Bud32.Nature communications · 2024Article
- Wide-ranging genetic variation in sensitivity to rapamycin in Drosophila melanogaster.Aging cell · 2024Article
- A highly conserved tRNA modification contributes toMicrobiology spectrum · 2024Article
- A versatile tRNA modification-sensitive northern blot method with enhanced performance.RNA (New York, N.Y.) · 2022Article
- p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes inFrontiers in cell and developmental biology · 2022Article
- Ionizing radiation and chemical oxidant exposure impacts on Cryptococcus neoformans transfer RNAs.PloS one · 2022Article
- The structural and functional workings of KEOPS.Nucleic acids research · 2021Review
- Variations in transfer and ribosomal RNA epitranscriptomic status can adapt eukaryote translation to changing physiological and environmental conditions.RNA biology · 2021Review
- Loss ofeLife · 2021Article
- Combining tRNA sequencing methods to characterize plant tRNA expression and post-transcriptional modification.RNA biology · 2021Article
- Regulation of Body Size and Growth Control.Genetics · 2020Review
- Naturally occurring modified ribonucleosides.Wiley interdisciplinary reviews. RNA · 2020Review
- Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase: Specificity determinants and tRNA-i6A37 profiles.PLoS genetics · 2020Article
- Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development.International journal of molecular sciences · 2020Article
- Article
- Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions.Science signaling · 2018Article
- Ras/ERK-signalling promotes tRNA synthesis and growth via the RNA polymerase III repressor Maf1 in Drosophila.PLoS genetics · 2018Article
- The tRNA biology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
N(6)-Threonylcarbamoyl-adenosine (t(6)A) is a universal modification occurring at position 37 in nearly all tRNAs that decode A-starting codons, including the eukaryotic initiator tRNA (tRNAi (Met)). Yeast lacking central components of the t(6)A synthesis machinery, such as Tcs3p (Kae1p) or Tcs5p (Bud32p), show slow-growth phenotypes. In the present work, we show that loss of the Drosophila tcs3 homolog also leads to a severe reduction in size and demonstrate, for the first time in a non-microbe, that Tcs3 is required for t(6)A synthesis. In Drosophila and in mammals, tRNAi (Met) is a limiting factor for cell and animal growth. We report that the t(6)A-modified form of tRNAi (Met) is the actual limiting factor. We show that changing the proportion of t(6)A-modified tRNAi (Met), by expression of an un-modifiable tRNAi (Met) or changing the levels of Tcs3, regulate target of rapamycin (TOR) kinase activity and influences cell and animal growth in vivo. These findings reveal an unprecedented relationship between the translation machinery and TOR, where translation efficiency, limited by the availability of t(6)A-modified tRNA, determines growth potential in eukaryotic cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.