ArticleAmerican journal of physiology. Cell physiology2015
Effect of denervation on the regulation of mitochondrial transcription factor A expression in skeletal muscle.
Article in American journal of physiology. Cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Adaptations in mitochondrial quality control and interactions with innate immune signaling within skeletal muscle: A narrative review.Journal of sport and health science · 2025Review
- Mitochondrial Biogenesis in Skeletal Muscle.Advances in experimental medicine and biology · 2025Review
- Mitochondrial Dysfunction in Intensive Care Unit-Acquired Weakness and Critical Illness Myopathy: A Narrative Review.International journal of molecular sciences · 2023Review
- P2 Receptor Signaling in Motor Units in Muscular Dystrophy.International journal of molecular sciences · 2023Review
- Resveratrol Blunts Mitochondrial Loss in Slow and Mixed Skeletal Muscle Phenotypes of Non-Human Primates following a Long-Term High Fat/Sugar Diet.Journal of dietary supplements · 2023Article
- Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Denervation induces mitochondrial decline and exacerbates lysosome dysfunction in middle-aged mice.Aging · 2022Article
- Mitochondrial Bioenergetics and Turnover during Chronic Muscle Disuse.International journal of molecular sciences · 2021Review
- PGC-1α overexpression partially rescues impaired oxidative and contractile pathophysiology following volumetric muscle loss injury.Scientific reports · 2019Article
- Mitochondrial dysfunction induces muscle atrophy during prolonged inactivity: A review of the causes and effects.Archives of biochemistry and biophysics · 2019Review
- Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.Physiological reports · 2018Article
- A mini review: Proteomics approaches to understand disused vs. exercised human skeletal muscle.Physiological genomics · 2018Review
- Denervation does not Induce Muscle Atrophy Through Oxidative Stress.European journal of translational myology · 2017Article
- Function of specialized regulatory proteins and signaling pathways in exercise-induced muscle mitochondrial biogenesis.Integrative medicine research · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to determine how the expression of mitochondrial transcription factor A (Tfam), a protein that governs mitochondrial DNA (mtDNA) transcription and replication, is regulated during a state of reduced organelle content imposed by muscle disuse. We measured Tfam expression at 8 h, 16 h, 24 h, 3 days, or 7 days following denervation and hypothesized that decreases in Tfam expression would precede mitochondrial loss. Muscle mass was lowered by 13% and 38% at 3 and 7 days postdenervation, while cytochrome c oxidase activity fell by 33% and 39% at the same time points. Tfam promoter activation in vivo was reduced by 30-65% between 8 h and 3 days of denervation, while Tfam transcript half-life was increased following 8-24 h of denervation. Protein expression of RNA-binding proteins that promote mRNA degradation (CUG repeat-binding protein and K homology splicing regulator protein) was elevated at 3 and 7 days of denervation. Tfam localization within subsarcolemmal mitochondria was reduced after 3 and 7 days of denervation and was associated with suppression of the cytochrome c oxidase type I transcript at 3 days, indicating that denervation impairs both mitochondrial Tfam import and mtDNA transcription during an early period following denervation. These data suggest that putative signals downregulate Tfam transcription during the earliest stages following denervation but are counteracted by increases in Tfam mRNA stability. Import of Tfam into the mitochondrion seems to be the most critical point of regulation of this protein during the early onset of denervation, an impairment of which is coincident with the loss of mitochondria during muscle disuse.
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