ArticleGenes & development2015
Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.
Article in Genes & development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 109 citations in OpenAlex.
- The influence of Polycomb and Trithorax group function on the sexually dimorphic abdomen pigmentation of Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- Polycomb Group Proteins inBiology · 2026Review
- Chromatin folding by the Polycomb group proteins and its elusive role in epigenetic repression.The FEBS journal · 2026Review
- VEL-dependent polymerization maintains the chromatin association of Polycomb proteins for the switch to epigenetic silencing.Molecular cell · 2025Article
- How CBX proteins regulate normal and leukemic blood cells.FEBS letters · 2024Review
- Polycomb protein binding and looping in the ON transcriptional state.Science advances · 2024Article
- Review
- DNA elements tether canonical Polycomb Repressive Complex 1 to human genes.Nucleic acids research · 2023Article
- Polycomb protein binding and looping mediated by Polycomb Response Elements in the ON transcriptional state.bioRxiv : the preprint server for biology · 2023Article
- You are who your friends are-nuclear pore proteins as components of chromatin-binding complexes.FEBS letters · 2023Review
- A bioinformatics screen reveals hox and chromatin remodeling factors at the Drosophila histone locus.BMC genomic data · 2023Article
- Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila.Genetics · 2023Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Genetic and Epigenetic Regulation ofJournal of developmental biology · 2023Review
- Polycomb group genes are required for neuronal pruning in Drosophila.BMC biology · 2023Article
- A bioinformatics screen reveals Hox and chromatin remodeling factors at thebioRxiv : the preprint server for biology · 2023Article
- Genetic variation in P-element dysgenic sterility is associated with double-strand break repair and alternative splicing of TE transcripts.PLoS genetics · 2022Article
- Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.Cell reports · 2022Article
- Regulation of Polyhomeotic Condensates by Intrinsically Disordered Sequences That Affect Chromatin Binding.Epigenomes · 2022Article
- Context-specific Polycomb mechanisms in development.Nature reviews. Genetics · 2022Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The Polycomb group (PcG) proteins are key regulators of development in Drosophila and are strongly implicated in human health and disease. How PcG complexes form repressive chromatin domains remains unclear. Using cross-linked affinity purifications of BioTAP-Polycomb (Pc) or BioTAP-Enhancer of zeste [E(z)], we captured all PcG-repressive complex 1 (PRC1) or PRC2 core components and Sex comb on midleg (Scm) as the only protein strongly enriched with both complexes. Although previously not linked to PRC2, we confirmed direct binding of Scm and PRC2 using recombinant protein expression and colocalization of Scm with PRC1, PRC2, and H3K27me3 in embryos and cultured cells using ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep sequencing). Furthermore, we found that RNAi knockdown of Scm and overexpression of the dominant-negative Scm-SAM (sterile α motif) domain both affected the binding pattern of E(z) on polytene chromosomes. Aberrant localization of the Scm-SAM domain in long contiguous regions on polytene chromosomes revealed its independent ability to spread on chromatin, consistent with its previously described ability to oligomerize in vitro. Pull-downs of BioTAP-Scm captured PRC1 and PRC2 and additional repressive complexes, including PhoRC, LINT, and CtBP. We propose that Scm is a key mediator connecting PRC1, PRC2, and transcriptional silencing. Combined with previous structural and genetic analyses, our results strongly suggest that Scm coordinates PcG complexes and polymerizes to produce broad domains of PcG silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.