ArticlePlant physiology2015
Analysis of Loss-of-Function Mutants in Aspartate Kinase and Homoserine Dehydrogenase Genes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 26 citations in OpenAlex.
- Advances in the Application of Amino Acids in the Preservation of Postharvest Fruits and Vegetables.Foods (Basel, Switzerland) · 2026Review
- Genome-wide identification of theFrontiers in plant science · 2026Article
- Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.Plant direct · 2025Article
- A mutation in THREONINE SYNTHASE 1 uncouples proliferation and transition domains of the root apical meristem: experimental evidence and in silico proposed mechanism.Development (Cambridge, England) · 2022Article
- New Insight into Aspartate Metabolic Pathways inInternational journal of molecular sciences · 2022Article
- Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-TolerantInternational journal of molecular sciences · 2021Article
- Functional relationships of three NFU proteins in the biogenesis of chloroplastic iron-sulfur clusters.Plant direct · 2021Article
- Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in thePlant direct · 2020Article
- Integrating transcriptomic network reconstruction and eQTL analyses reveals mechanistic connections between genomic architecture and Brassica rapa development.PLoS genetics · 2019Article
- An L,L-diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis.Journal of experimental botany · 2018Article
- Whole-transcriptome RNA-seq, gene set enrichment pathway analysis, and exon coverage analysis of two plastid RNA editing mutants.Plant signaling & behavior · 2017Article
- An Organelle RNA Recognition Motif Protein Is Required for Photosystem II SubunitPlant physiology · 2017Article
- Chloroplastic iron-sulfur scaffold protein NFU3 is essential to overall plant fitness.Plant signaling & behavior · 2017Article
- Network-Guided GWAS Improves Identification of Genes Affecting Free Amino Acids.Plant physiology · 2017Article
- A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins.Plant physiology · 2016Article
- Genome-Wide Analysis of the Lysine Biosynthesis Pathway Network during Maize Seed Development.PloS one · 2016Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosynthesis of aspartate (Asp)-derived amino acids lysine (Lys), methionine (Met), threonine (Thr), and isoleucine involves monofunctional Asp kinases (AKs) and dual-functional Asp kinase-homoserine dehydrogenases (AK-HSDHs). Four-week-old loss-of-function Arabidopsis (Arabidopsis thaliana) mutants in the AK-HSDH2 gene had increased amounts of Asp and Asp-derived amino acids, especially Thr, in leaves. To explore mechanisms behind this phenotype, we obtained single mutants for other AK and AK-HSDH genes, generated double mutants from ak-hsdh2 and ak mutants, and performed free and protein-bound amino acid profiling, transcript abundance, and activity assays. The increases of Asp, Lys, and Met in ak-hsdh2 were also observed in ak1-1, ak2-1, ak3-1, and ak-hsdh1-1. However, the Thr increase in ak-hsdh2 was observed in ak-hsdh1-1 but not in ak1-1, ak2-1, or ak3-1. Activity assays showed that AK2 and AK-HSDH1 are the major contributors to overall AK and HSDH activities, respectively. Pairwise correlation analysis revealed positive correlations between the amount of AK transcripts and Lys-sensitive AK activity and between the amount of AK-HSDH transcripts and both Thr-sensitive AK activity and total HSDH activity. In addition, the ratio of total AK activity to total HSDH activity negatively correlates with the ratio of Lys to the total amount of Met, Thr, and isoleucine. These data led to the hypothesis that the balance between Lys-sensitive AKs and Thr-sensitive AK-HSDHs is important for maintaining the amounts and ratios of Asp-derived amino acids.
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