ArticleDevelopment (Cambridge, England)2015
Wnt signaling and tbx16 form a bistable switch to commit bipotential progenitors to mesoderm.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Tbx16 and mesogenin 1 promote presomitic mesoderm differentiation by repressing the mesodermal progenitor cell state.PLoS genetics · 2026Article
- The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential.Development (Cambridge, England) · 2025Article
- Cell type-independent timekeeping gene modules enable embryonic stage prediction in zebrafish.bioRxiv : the preprint server for biology · 2025Article
- The physical roles of different posterior tissues in zebrafish axis elongation.Nature communications · 2025Article
- Spinal cord elongation enables proportional regulation of the zebrafish posterior body.Development (Cambridge, England) · 2025Article
- Approximated gene expression trajectories for gene regulatory network inference on cell tracks.iScience · 2024Article
- On the evolutionary origin of discrete phenotypic plasticity.G3 (Bethesda, Md.) · 2024Article
- Origin, heterogeneity, and interconversion of noncanonical bistable switches from the positive feedback loops under dual signaling.iScience · 2023Article
- An ancestral Wnt-Brachyury feedback loop in axial patterning and recruitment of mesoderm-determining target genes.Nature ecology & evolution · 2022Article
- Article
- Mesoderm induction and patterning: Insights from neuromesodermal progenitors.Seminars in cell & developmental biology · 2022Review
- The unappreciated generative role of cell movements in pattern formation.Royal Society open science · 2022Review
- Regulation of stem cell identity by miR-200a during spinal cord regeneration.Development (Cambridge, England) · 2022Article
- Identification of in vivo Hox13-binding sites reveals an essential locus controlling zebrafish brachyury expression.Development (Cambridge, England) · 2021Article
- Overview of Secondary Neurulation.Journal of Korean Neurosurgical Society · 2021Article
- Hox13 genes are required for mesoderm formation and axis elongation during early zebrafish development.Development (Cambridge, England) · 2020Article
- Fgf-driven Tbx protein activities directly induceDevelopment (Cambridge, England) · 2020Article
- Neuromesodermal Progenitors: A Basis for Robust Axial Patterning in Development and Evolution.Frontiers in cell and developmental biology · 2020Review
- Article
- Tris(1,3-dichloro-2-propyl) Phosphate Exposure During the Early-Blastula Stage Alters the Normal Trajectory of Zebrafish Embryogenesis.Environmental science & technology · 2018Article
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7 authors.
Funding
Abstract
Anterior to posterior growth of the vertebrate body is fueled by a posteriorly located population of bipotential neuro-mesodermal progenitor cells. These progenitors have a limited rate of proliferation and their maintenance is crucial for completion of the anterior-posterior axis. How they leave the progenitor state and commit to differentiation is largely unknown, in part because widespread modulation of factors essential for this process causes organism-wide effects. Using a novel assay, we show that zebrafish Tbx16 (Spadetail) is capable of advancing mesodermal differentiation cell-autonomously. Tbx16 locks cells into the mesodermal state by not only activating downstream mesodermal genes, but also by repressing bipotential progenitor genes, in part through a direct repression of sox2. We demonstrate that tbx16 is activated as cells move from an intermediate Wnt environment to a high Wnt environment, and show that Wnt signaling activates the tbx16 promoter. Importantly, high-level Wnt signaling is able to accelerate mesodermal differentiation cell-autonomously, just as we observe with Tbx16. Finally, because our assay for mesodermal commitment is quantitative we are able to show that the acceleration of mesodermal differentiation is surprisingly incomplete, implicating a potential separation of cell movement and differentiation during this process. Together, our data suggest a model in which high levels of Wnt signaling induce a transition to mesoderm by directly activating tbx16, which in turn acts to irreversibly flip a bistable switch, leading to maintenance of the mesodermal fate and repression of the bipotential progenitor state, even as cells leave the initial high-Wnt environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.