ArticleScientific reports2015
RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- ZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement.Scientific reports · 2019Article
- Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins.BMC biotechnology · 2016Article
- Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications.Scientific reports · 2016Article
- Bacterial community composition of an urban river in the North West Province, South Africa, in relation to physico-chemical water quality.Environmental science and pollution research international · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The ability to assemble DNA sequences de novo through efficient and powerful DNA fabrication methods is one of the foundational technologies of synthetic biology. Gene synthesis, in particular, has been considered the main driver for the emergence of this new scientific discipline. Here we describe RapGene, a rapid gene assembly technique which was successfully tested for the synthesis and cloning of both prokaryotic and eukaryotic genes through a ligation independent approach. The method developed in this study is a complete bacterial gene synthesis platform for the quick, accurate and cost effective fabrication and cloning of gene-length sequences that employ the widely used host Escherichia coli.
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Registered trials
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