ArticleBMC developmental biology2015
Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos.
Article in BMC developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Identification of stable miRNA references for qRT-PCR normalization in ovine fasciolosis.BMC genomics · 2026Article
- Expression of miRNAs Associated With Embryo Development and DNA Damage Response in Porcine Embryos.Molecular reproduction and development · 2025Article
- Article
- microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus).Molecular reproduction and development · 2025Article
- Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media.Journal of developmental biology · 2023Article
- Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review.Life (Basel, Switzerland) · 2023Review
- Article
- Berberine regulates lipid metabolism via miR-192 in porcine oocytes matured in vitro.Veterinary medicine and science · 2021Article
- Murine Blastocysts Release Mature MicroRNAs Into Culture Media That Reflect Developmental Status.Frontiers in genetics · 2021Article
- Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos.Animals : an open access journal from MDPI · 2020Article
- Identification of suitable controls for miRNA quantification in T-cells and whole blood cells in sepsis.Scientific reports · 2019Article
- Identification of endogenous microRNA references in porcine serum for quantitative real-time PCR normalization.Molecular biology reports · 2018Article
- A three-microRNA signature for lung squamous cell carcinoma diagnosis in Chinese male patients.Oncotarget · 2017Article
- Effect ofAsian-Australasian journal of animal sciences · 2017Article
- A comprehensive method for identification of suitable reference genes in extracellular vesicles.Journal of extracellular vesicles · 2017Article
- Article
- Differential expression of circulating microRNAs in blood and haematoma samples from patients with intracerebral haemorrhage.The Journal of international medical research · 2016Article
- Identification of the MicroRNA Repertoire in TLR-Ligand Challenged Bubaline PBMCs as a Model of Bacterial and Viral Infection.PloS one · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are small noncoding RNAs that act as post-transcriptional regulators of gene targets. Accurate quantification of miRNA expression using validated internal controls should aid in the understanding of their role in epigenetic modification of genome function. To date, most studies that have examined miRNA expression levels have used the global mean expression of all expressed genes or the expression of reference mRNAs or nuclear RNAs for normalization.
resultsWe analyzed the suitability of a number of miRNAs as potential expression normalizers in bovine oocytes and early embryos, and porcine oocytes. The stages examined were bovine oocytes at the germinal vesicle (GV) and metaphase II stages, bovine zygotes, 2, 4 and 8 cell embryos, morulae and blastocysts, as well as porcine cumulus oocyte complexes, GV, metaphase I and II oocytes. qRT-PCR was performed to quantify expression of miR-93, miR-103, miR-26a, miR-191, miR-23b, Let-7a and U6 for bovine samples and miR-21, miR-26a, miR-93, miR-103, miR-148a, miR-182 and miR-191 for porcine oocytes. The average starting material for each sample was determined using specific standard curves for each primer set. Subsequently, geNorm and BestKeeper software were used to identify a set of stably expressed miRNAs. Stepwise removal to determine the optimum number of reference miRNAs identified miR-93 and miR-103 as the most stably expressed in bovine samples and miR-26a, miR-191 and miR-93 in porcine samples.
conclusionsThe combination of miR-93 and miR-103 is optimal for normalizing miRNA expression for qPCR experiments on bovine oocytes and preimplantation embryos; the preferred combination for porcine oocytes is miR-26a, miR-191 and miR-93.
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