Evidence map›Paper›PMID 26062606›Full record

ArticleParasites & vectors2015

Vector sequence contamination of the Plasmodium vivax sequence database in PlasmoDB and In silico correction of 26 parasite sequences.

Zhi-Yong Tao, Xu Sui, Cao Jun, Richard Culleton, Qiang Fang, Hui Xia, Qi Gao

Abstract readLetter
In one paragraph

Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zhi-Yong TaoDepartment of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. Bengbucity@163.com.
Xu SuiJiangsu Institute of Parasitic Diseases, Wuxi, China. uouping7@gmail.com.
Cao JunJiangsu Institute of Parasitic Diseases, Wuxi, China. caojuncn@hotmail.com.
Richard CulletonMalaria Unit, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan. richard.culleton@gmail.com.
Qiang FangDepartment of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. fq333@sohu.com.
Hui XiaDepartment of Parasitology, Bengbu Medical College, Bengbu, People's Republic of China. xiahui912@163.com.
Qi GaoJiangsu Institute of Parasitic Diseases, Wuxi, China. gaoqi54@hotmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We found a 47 aa protein sequence that occurs 17 times in the Plasmodium vivax nucleotide database published on PlasmoDB. Coding sequence analysis showed multiple restriction enzyme sites within the 141 bp nucleotide sequence, and a His6 tag attached to the 3' end, suggesting cloning vector origins. Sequences with vector contamination were submitted to NCBI, and BLASTN was used to cross-examine whole-genome shotgun contigs (WGS) from four recently deposited P. vivax whole genome sequencing projects. There are at least 26 genes listed in the PlasmoDB database that incorporate this cloning vector sequence into their predicted provisional protein products.

Indexed as

Databases, Nucleic AcidAnimalsBase SequenceComputer SimulationGenetic VectorsGenome, ProtozoanMolecular Sequence DataPlasmodium vivax

Identifiers

PMID26062606
PMCPMC4464627

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.