ArticlePloS one2015
A Defensin from the Model Beetle Tribolium castaneum Acts Synergistically with Telavancin and Daptomycin against Multidrug Resistant Staphylococcus aureus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Edible Insects as Future Proteins: Nutritional Value, Functional Properties, Bioactivities, and Safety Perspectives.Nutrients · 2025Review
- Pattern recognition, hemolymph protease-14 activation, and enhancement of lysozyme-mediated bacteria killing by soluble peptidoglycan recognition proteins in Manduca sexta.Insect biochemistry and molecular biology · 2025Article
- Biological Function of Antimicrobial Peptides on Suppressing Pathogens and Improving Host Immunity.Antibiotics (Basel, Switzerland) · 2023Review
- Insect Antimicrobial Peptides: Advancements, Enhancements and New Challenges.Antibiotics (Basel, Switzerland) · 2023Review
- Process intensification for the continuous production of an antimicrobial peptide in stably-transformed Sf-9 insect cells.Scientific reports · 2022Article
- Tribolium castaneum defensin 1 kills Moraxella catarrhalisin an in vitro infection model but does not harm commensal bacteria.Virulence · 2021Article
- Article
- Repurposing Kinase Inhibitor Bay 11-7085 to CombatFrontiers in pharmacology · 2021Article
- New Antimicrobial Bioactivity against Multidrug-Resistant Gram-Positive Bacteria of Kinase Inhibitor IMD0354.Antibiotics (Basel, Switzerland) · 2020Article
- Antibacterial activity of aVirulence · 2019Article
- Natural Occurrence in Venomous Arthropods of Antimicrobial Peptides Active against Protozoan Parasites.Toxins · 2019Review
- Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies - A Review.Frontiers in physiology · 2019Review
- Antimicrobial activity of 1,3,4-oxadiazole derivatives against planktonic cells and biofilm of Staphylococcus aureus.Future medicinal chemistry · 2018Article
- Article
- Characterization of a Francisella tularensis-Caenorhabditis elegans Pathosystem for the Evaluation of Therapeutic Compounds.Antimicrobial agents and chemotherapy · 2017Article
- Synergistic Efficacy of Aedes aegypti Antimicrobial Peptide Cecropin A2 and Tetracycline against Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2017Article
- Structure-Activity Relationships of Insect Defensins.Frontiers in chemistry · 2017Review
- Insect antimicrobial peptides act synergistically to inhibit a trypanosome parasite.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Article
- Diversity, evolution and medical applications of insect antimicrobial peptides.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Antimicrobial Peptides as Potential Alternatives to Antibiotics in Food Animal Industry.International journal of molecular sciences · 2016Review
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
The red flour beetle Tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. This study demonstrates that defensin 1 from T. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant Staphylococcus aureus strains. The minimum inhibitory concentration (MIC) of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. The putative mode of action of the defensin peptide is disruption of the bacterial cell membrane. The antibacterial activity of defensin 1 was attenuated by salt concentrations of 1.56 mM and 25 mM for NaCl and CaCl2 respectively. Treatment of defensin 1 with the reducing agent dithiothreitol (DTT) at concentrations 1.56 to 3.13 mM abolished the antimicrobial activity of the peptide. In the presence of subinhibitory concentrations of antibiotics that also target the bacterial cell envelope such as telavancin and daptomycin, the MIC of the peptide was as low as 1 μg/ml. Moreover, when tested against an S. aureus strain that was defective in D-alanylation of the cell wall, the MIC of the peptide was 0.5 μg/ml. Defensin 1 exhibited no toxicity against human erythrocytes even at 400 μg/ml. The in vivo activity of the peptide was validated in a Caenorhabditis elegans-MRSA liquid infection assay. These results suggest that defensin 1 behaves similarly to other cationic AMPs in its mode of action against S. aureus and that the activity of the peptide can be enhanced in combination with other antibiotics with similar modes of action or with compounds that have the ability to decrease D-alanylation of the bacterial cell wall.
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