Evidence map›Paper›PMID 26062100›Full record

ArticleThe journal of sexual medicine2015

Intratunical Injection of Genetically Modified Adipose Tissue-Derived Stem Cells with Human Interferon α-2b for Treatment of Erectile Dysfunction in a Rat Model of Tunica Albugineal Fibrosis.

Ahmet Gokce, Zakaria Y Abd Elmageed, George F Lasker, Mostafa Bouljihad, Stephen E Braun, Hogyoung Kim, Philip J Kadowitz, Asim B Abdel-Mageed, Suresh C Sikka, Wayne J Hellstrom

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In one paragraph

Article in The journal of sexual medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Ahmet GokceDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
Zakaria Y Abd ElmageedDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
George F LaskerDepartment of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA.
Mostafa BouljihadTulane National Primate Research Center, New Orleans, LA, USA.
Stephen E BraunTulane National Primate Research Center, New Orleans, LA, USA.
Hogyoung KimDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
Philip J KadowitzDepartment of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA.
Asim B Abdel-MageedDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
Suresh C SikkaDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
Wayne J HellstromDepartment of Urology, Tulane University School of Medicine, New Orleans, LA, USA.
Tulane University · USUniversity of New Orleans · US

Funding

Tulane NPRC SPF Sheltered Outdoor Enclosure ExpansionP51OD011104 · OD · TULANE UNIVERSITY OF LOUISIANA · PI L Lee HAMM · 2012 to 2026
$142.4M
NIH HHS P51 OD011104
6 · The paper itself

Abstract

introductionPeyronie's disease (PD) has frequently been associated with erectile dysfunction (ED) and may further compromise coitus.

aimTo investigate the efficacy of intratunical injection of genetically modified rat adipose tissue-derived stem cells (ADSCs) expressing human interferon α-2b (ADSCs-IFN) in decreasing fibrosis and restoring erectile function in a rat model of tunica albugineal fibrosis (TAF).

methodsA total of 36 Sprague-Dawley rats (12 weeks old; 300-350 g) were randomly divided in six equal groups: (i) sham group (50 μL saline-injected into the tunica albuginea [TA]); (ii) TAF group (transforming growth factor [TGF]-β1 [0.5 μg/50 μL] injected into the TA); (iii) TGF-β1 plus 5 × 10(5) control ADSCs injected same day; (iv) TGF-β1 plus 5 × 10(5) ADSCs-IFN injected same day; (v) TGF-β1 plus 5 × 10(5) control ADSCs injected after 30 days; and (vi) TGF-β1 plus 5 × 10(5) ADSCs-IFN injected after 30 days. Rat allogeneic ADSCs were harvested from inguinal fat tissue.

main outcome measuresForty-five days following the TGF-β1 injection, erectile function was assessed, and penile tissues were harvested for further evaluations.

resultsIn the same-day injection groups, intratunical injection of ADSCs and ADSC-IFN improved erectile response observed upon stimulation of cavernous nerve compared with TAF group. Intratunical ADSC-IFN injection at day 30 improved erectile responses 3.1, 1.8, and 1.3 fold at voltages of 2.5, 5.0, and 7.0, respectively, when compared with TAF group. Furthermore, at voltages of 2.5 and 5.0, treatment on day 30 with ADSCs-IFN improved erectile responses 1.6- and 1.3-fold over treatment with ADSCs alone. Local injection of ADSCs or ADSCs-IFN reduced Peyronie's-like manifestations, and these effects might be associated with a decrease in the expression of tissue inhibitors of metalloproteinases.

conclusionThis study documents that transplantation of genetically modified ADSCs, with or without human IFN α-2b, attenuated Peyronie's-like changes and enhanced erectile function in a rat model of TAF.

Indexed as

Stem Cell TransplantationAdipose TissueAnimalsDisease Models, AnimalErectile DysfunctionFibrosisHumansInjections, IntralesionalInterferon-alphaInterferon alpha-2MalePenile IndurationPenisRatsRats, Sprague-DawleyRecombinant ProteinsInterferon-alphaInterferon alpha-2Recombinant ProteinsErectile DysfunctionFibrosisGene TherapyHuman Interferon α-2bPeyronie's DiseaseStem CellsTunica Albuginea

Identifiers

PMID26062100
PMCPMC4914049
OpenAlexW1563514202

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.