ArticleAutophagy2015
A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis.
Article in Autophagy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- General autophagy-dependent and -independent lipophagic processes collaborate to regulate the overall level of lipophagy in yeast.Autophagy · 2024Article
- Automated quantification of vacuole fusion and lipophagy inAutophagy · 2024Article
- Lipid Droplets in EndosymbioticPlants (Basel, Switzerland) · 2024Review
- A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast.FEMS yeast research · 2024Article
- Identification and Potential Participation of Lipases in Autophagic Body Degradation in Embryonic Axes of Lupin (International journal of molecular sciences · 2023Article
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- Topology and Function of theCells · 2023Article
- Shared genetic loci for body fat storage and adipocyte lipolysis in humans.Scientific reports · 2022Article
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- How Cells Deal with the Fluctuating Environment: Autophagy Regulation under Stress in Yeast and Mammalian Systems.Antioxidants (Basel, Switzerland) · 2022Review
- Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.Autophagy · 2021Article
- Lipid Droplets and Their Autophagic Turnover via the Raft-Like Vacuolar Microdomains.International journal of molecular sciences · 2021Review
- The Redox Proteome of Thiol Proteins in the Rice Blast FungusFrontiers in microbiology · 2021Article
- Completing Autophagy: Formation and Degradation of the Autophagic Body and Metabolite Salvage in Plants.International journal of molecular sciences · 2020Review
- Degradation of Lipid Droplets in Plants and Algae-Right Time, Many Paths, One Goal.Frontiers in plant science · 2020Review
- Spatial distribution of lipid droplets during starvation: Implications for lipophagy.Communicative & integrative biologyArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid droplets (LDs) are the conserved organelles for the deposit of neutral lipids, and function as reservoirs of membrane and energy sources. To date, functional links between autophagy and LD dynamics have not been fully elucidated. Here, we report that a vacuolar putative lipase, Atg15, required for degradation of autophagic bodies, is crucial for the maintenance of LD amount in the yeast Saccharomyces cerevisiae in the stationary phase. Mutant analyses revealed that the putative lipase motif and vacuolar localization of Atg15 are important for the maintenance of LD amount. Loss of autophagosome formation by simultaneous deletion of core ATG genes cancelled the reduction in the LD amount in ATG15-deleted cells, indicating that degradation of autophagic bodies accounts for the functional involvement of Atg15 in LD dynamics. The reduced level of LDs in the mutant strain was dependent on Tgl3 and Tgl4, major lipases for lipolysis in S. cerevisiae. An altered phosphorylation status of Tgl3, higher accumulation of Tgl4, and closer associations of Tgl3 and Tgl4 with LDs were detected in the ATG15-deleted cells. Furthermore, increased levels of downstream metabolites of lipolysis in the mutant strain strongly suggested enhanced lipolytic activity caused by loss of ATG15. Our data provide evidence for a novel link between autophagic flux and LD dynamics integrated with Atg15 activity.
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