ArticlePloS one2015
Generation of Brain Microvascular Endothelial-Like Cells from Human Induced Pluripotent Stem Cells by Co-Culture with C6 Glioma Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- A Long-Lived Human Neurovascular PENTA Culture Model Captures Incomplete Vascular Repair and Glia-Associated Signaling After Traumatic Brain Injury.Advanced healthcare materials · 2026Article
- Human bone marrow-mesenchymal stem cells differentiation into brain-like endothelial cells.Turkish journal of biology = Turk biyoloji dergisi · 2026Article
- Co-Culture Models: Key Players in In Vitro Neurotoxicity, Neurodegeneration and BBB Modeling Studies.Biomedicines · 2024Review
- Construction and Functional Evaluation of a Three-Dimensional Blood-Brain Barrier Model Equipped With Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells.Pharmaceutical research · 2022Article
- Functional analysis of human brain endothelium using a microfluidic device integrating a cell culture insert.APL bioengineering · 2022Article
- Article
- iPSC-Derived Liver Organoids: A Journey from Drug Screening, to Disease Modeling, Arriving to Regenerative Medicine.International journal of molecular sciences · 2020Review
- Strategies for derivation of endothelial lineages from human stem cells.Stem cell research & therapy · 2019Review
- Neurovascular Organotypic Culture Models Using Induced Pluripotent Stem Cells to Assess Adverse Chemical Exposure Outcomes.Applied in vitro toxicology · 2019Article
- Preparation of iPS cell-derived CD31Regenerative therapy · 2018Article
- Establishment of a Human iPSC- and Nanofiber-Based Microphysiological Blood-Brain Barrier System.ACS applied materials & interfaces · 2018Article
- ETV2-TET1/TET2 Complexes Induce Endothelial Cell-Specific Robo4 Expression via Promoter Demethylation.Scientific reports · 2018Article
- Stem Cells as a Promising Tool for the Restoration of Brain Neurovascular Unit and Angiogenic Orientation.Molecular neurobiology · 2017Review
- Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells.Science advances · 2017Article
- Connexin Channels at the Glio-Vascular Interface: Gatekeepers of the Brain.Neurochemical research · 2017Review
- Stem cell models of Alzheimer's disease: progress and challenges.Alzheimer's research & therapy · 2017Review
- In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.Stem cell reports · 2017Article
- The Microvascular Gap Junction Channel: A Route to Deliver MicroRNAs for Neurological Disease Treatment.Frontiers in molecular neuroscience · 2017Article
- Translational Prospects and Challenges in Human Induced Pluripotent Stem Cell Research in Drug Discovery.Cells · 2016Review
- Modeling Alzheimer's disease with human induced pluripotent stem (iPS) cells.Molecular and cellular neurosciences · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The blood brain barrier (BBB) is formed by brain microvascular endothelial cells (BMECs) and tightly regulates the transport of molecules from blood to neural tissues. In vitro BBB models from human pluripotent stem cell (PSCs)-derived BMECs would be useful not only for the research on the BBB development and function but also for drug-screening for neurological diseases. However, little is known about the differentiation of human PSCs to BMECs. In the present study, human induced PSCs (iPSCs) were differentiated into endothelial cells (ECs), and further maturated to BMECs. Interestingly, C6 rat glioma cell-conditioned medium (C6CM), in addition to C6 co-culture, induced the differentiation of human iPSC-derived ECs (iPS-ECs) to BMEC-like cells, increase in the trans-endothelial electrical resistance, decreased in the dextran transport and up-regulation of gene expression of tight junction molecules in human iPS-ECs. Moreover, Wnt inhibitors attenuated the effects of C6CM. In summary, we have established a simple protocol of the generation of BMEC-like cells from human iPSCs, and have demonstrated that differentiation of iPS-ECs to BMEC-like cells is induced by C6CM-derived signals, including canonical Wnt signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.