ArticlePloS one2015
Mechanisms of Toxicity of Ag Nanoparticles in Comparison to Bulk and Ionic Ag on Mussel Hemocytes and Gill Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 147 citations in OpenAlex.
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- Antitumoral efficacy of silver nanoparticles reduced with β-D-glucose on a canine transmissible venereal tumor cell line.Frontiers in veterinary science · 2026Article
- Structural, Adsorptive, and Antibacterial Properties of a Novel Silver (Diethyldithiocarbamate)-Decorated Reduced Graphene Oxide Nanocomposite for Sustainable Wastewater Treatment.Nanomaterials (Basel, Switzerland) · 2025Article
- Toxicity analysis of gold nanoparticle formulations used for intraocular applications.Scientific reports · 2025Article
- From Chromosomal Aberrations to Transcriptome Analysis: Four Decades of Research in Bivalve Genotoxicity.International journal of molecular sciences · 2025Review
- Article
- The use of nanoparticles in the treatment of infectious diseases and cancer, dental applications and tissue regeneration: a review.Frontiers in medical technology · 2023Review
- pH-Responsive PVA-Based Nanofibers Containing GO Modified with Ag Nanoparticles: Physico-Chemical Characterization, Wound Dressing, and Drug Delivery.Micromachines · 2022Article
- Curcumin Sustained Release with a Hybrid Chitosan-Silk Fibroin Nanofiber Containing Silver Nanoparticles as a Novel Highly Efficient Antibacterial Wound Dressing.Nanomaterials (Basel, Switzerland) · 2022Article
- Recent Advances in Metal-Based Nanoparticle-Mediated Biological Effects inMaterials (Basel, Switzerland) · 2022Review
- Ag-Contained Superabsorbent Curdlan-Chitosan Foams for Healing Wounds in a Type-2 Diabetic Mice Model.Pharmaceutics · 2022Article
- Effect of silver nanoparticles on gene transcription of land snail Helix aspersa.Scientific reports · 2022Article
- Zn-Mg-WC Nanocomposites for Bioresorbable Cardiovascular Stents: Microstructure, Mechanical Properties, Fatigue, Shelf Life, and Corrosion.ACS biomaterials science & engineering · 2022Article
- Carboxymethyl Dextran-Based Nanomicelle Coatings on Microarc Oxidized Titanium Surface for Percutaneous Implants: Drug Release, Antibacterial Properties, and Biocompatibility.BioMed research international · 2022Article
- Building the Bridge From Aquatic Nanotoxicology to Safety by Design Silver Nanoparticles.Frontiers in bioengineering and biotechnology · 2022Review
- Methodological Approaches To Assess Innate Immunity and Innate Memory in Marine Invertebrates and Humans.Frontiers in toxicology · 2022Review
- Aquatic organisms modulate the bioreactivity of engineered nanoparticles: focus on biomolecular corona.Frontiers in toxicology · 2022Review
- Eco-Interactions of Engineered Nanomaterials in the Marine Environment: Towards an Eco-Design Framework.Nanomaterials (Basel, Switzerland) · 2021Review
- Cross-Species Comparisons of Nanoparticle Interactions with Innate Immune Systems: A Methodological Review.Nanomaterials (Basel, Switzerland) · 2021Review
- Functional and Morphological Changes Induced inNanomaterials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silver nanoparticles (Ag NPs) are increasingly used in many products and are expected to end up in the aquatic environment. Mussels have been proposed as marine model species to evaluate NP toxicity in vitro. The objective of this work was to assess the mechanisms of toxicity of Ag NPs on mussel hemocytes and gill cells, in comparison to ionic and bulk Ag. Firstly, cytotoxicity of commercial and maltose stabilized Ag NPs was screened in parallel with the ionic and bulk forms at a wide range of concentrations in isolated mussel cells using cell viability assays. Toxicity of maltose alone was also tested. LC50 values were calculated and the most toxic Ag NPs tested were selected for a second step where sublethal concentrations of each Ag form were tested using a wide array of mechanistic tests in both cell types. Maltose-stabilized Ag NPs showed size-dependent cytotoxicity, smaller (20 nm) NPs being more toxic than larger (40 and 100 nm) NPs. Maltose alone provoked minor effects on cell viability. Ionic Ag was the most cytotoxic Ag form tested whereas bulk Ag showed similar cytotoxicity to the commercial Ag NPs. Main mechanisms of action of Ag NPs involved oxidative stress and genotoxicity in the two cell types, activation of lysosomal AcP activity, disruption of actin cytoskeleton and stimulation of phagocytosis in hemocytes and increase of MXR transport activity and inhibition of Na-K-ATPase in gill cells. Similar effects were observed after exposure to ionic and bulk Ag in the two cell types, although generally effects were more marked for the ionic form. In conclusion, results suggest that most observed responses were due at least in part to dissolved Ag.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.