ArticleMemorias do Instituto Oswaldo Cruz2015
A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines.
Article in Memorias do Instituto Oswaldo Cruz, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Submicroscopic infections and two malaria vectors are major contributors to residual malaria in an Amazonian village in Peru.Scientific reports · 2025Article
- First detection of Nyssorhynchus rondoniensis (Diptera: Culicidae) in southern Venezuela and its infection with Plasmodium falciparum.Acta tropica · 2025Article
- Forest cover percentage drives the peak biting time of Nyssorhynchus darlingi (Diptera: Culicidae) in the Brazilian Amazon.Malaria journal · 2024Article
- Effect of spatiotemporal variables on abundance, biting activity and parity of Nyssorhynchus darlingi (Diptera: Culicidae) in peri-Iquitos, Peru.Malaria journal · 2024Article
- Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil.Microorganisms · 2023Article
- Natural Infection of Nyssorhynchus darlingi and Nyssorhynchus benarrochi B with Plasmodium during the Dry Season in the Understudied Low-Transmission Setting of Datem del Marañon Province, Amazonian Peru.The American journal of tropical medicine and hygiene · 2023Article
- Using qPCR to compare the detection ofFrontiers in parasitology · 2023Article
- Anopheles (Kerteszia) cruzii infected by Plasmodium in the Atlantic Forest indicates that the malaria transmission cycle is maintained even after howler monkeys' population decline.Parasitology research · 2022Article
- Susceptibility of Field-CollectedGenes · 2021Article
- Vector role and human biting activity of Anophelinae mosquitoes in different landscapes in the Brazilian Amazon.Parasites & vectors · 2021Article
- Complexity of malaria transmission dynamics in the Brazilian Atlantic Forest.Current research in parasitology & vector-borne diseases · 2021Article
- Higher risk of malaria transmission outdoors than indoors by Nyssorhynchus darlingi in riverine communities in the Peruvian Amazon.Parasites & vectors · 2019Article
- Vector competence, vectorial capacity of Nyssorhynchus darlingi and the basic reproduction number of Plasmodium vivax in agricultural settlements in the Amazonian Region of Brazil.Malaria journal · 2019Article
- Nyssorhynchus dunhami: bionomics and natural infection by Plasmodium falciparum and P. vivax in the Peruvian Amazon.Memorias do Instituto Oswaldo Cruz · 2018Article
- Decreasing proportion of Anopheles darlingi biting outdoors between long-lasting insecticidal net distributions in peri-Iquitos, Amazonian Peru.Malaria journal · 2018Article
- Intensive trapping of blood-fed Anopheles darlingi in Amazonian Peru reveals unexpectedly high proportions of avian blood-meals.PLoS neglected tropical diseases · 2017Article
- Epidemic and Non-Epidemic Hot Spots of Malaria Transmission Occur in Indigenous Comarcas of Panama.PLoS neglected tropical diseases · 2016Article
- Article
- Evidence for temporal population replacement and the signature of ecological adaptation in a major Neotropical malaria vector in Amazonian Peru.Malaria journal · 2015Article
- The risk of malaria infection for travelers visiting the Brazilian Amazonian region: A mathematical modeling approach.Travel medicine and infectious diseaseArticle
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Authors and funding
6 authors.
Funding
Abstract
We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.
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